Improving Ovine Behavioral Pain Diagnosis by Implementing Statistical Weightings Based on Logistic Regression and Random Forest Algorithms

Author(s)

Esteves Trindade, Pedro Henrique ; Serrajordia Rocha de Mello, Joao Fernando ; Oliveira Figueiredo Silva, Nuno Emanuel ; Loureiro Luna, Stelio Pacca

• Document type: Journal article
• Source: ANIMALS; v. 12, n. 21, p. 19-pg., 2022-11-01.

Abstract

Simple Summary After four decades of studies on methods to assess pain in sheep, a pain scale composed of behavioral items that are fast, robust, and simple to apply was recently developed-the Unesp-Botucatu sheep acute pain scale (USAPS). Scientific evidence suggests that considering the importance of each behavior separately may improve the quality of pain diagnosis; however, this has not yet been studied for animal pain assessment. Therefore, the objective of this study was to investigate whether the implementation of statistical weights using machine learning algorithms improves the discriminatory capacity of the USAPS. A behavioral database, previously collected for USAPS validation, of 48 sheep before and after an abdominal surgical procedure was used. A multilevel binomial logistic regression algorithm and a random forest algorithm were used to establish the statistical weights and classify the sheep as to whether they needed analgesia or not. The quality of the USAPS pain diagnosis weighted by the two algorithms was better than the original version of the instrument. We conclude that considering the importance of each USAPS behavior by the two machine learning algorithms improved the instrument's ability to differentiate sheep in pain from those free of pain. Recently, the Unesp-Botucatu sheep acute pain scale (USAPS) was created, refined, and psychometrically validated as a tool that offers fast, robust, and simple application. Evidence points to an improvement in pain diagnosis when the importance of the behavioral items of an instrument is statistically weighted; however, this has not yet been investigated in animals. The objective was to investigate whether the implementation of statistical weightings using machine learning algorithms improves the USAPS discriminatory capacity. A behavioral database, previously collected for USAPS validation, of 48 sheep in the perioperative period of laparoscopy was used. A multilevel binomial logistic regression algorithm and a random forest algorithm were used to determine the statistical weights and classify the sheep as to whether they needed analgesia or not. The quality of the classification, estimated by the area under the curve (AUC) and its 95% confidence interval (CI), was compared between the USAPS versions. The USAPS AUCs weighted by multilevel binomial logistic regression (96.59 CI: [95.02-98.15]; p = 0.0004) and random forest algorithms (96.28 CI: [94.17-97.85]; p = 0.0067) were higher than the original USAPS AUC (94.87 CI: [92.94-96.80]). We conclude that the implementation of statistical weights by the two machine learning algorithms improved the USAPS discriminatory ability. (AU)

• FAPESP's process: 17/12815-0 - Pain and quality of life in animals
• Grantee: Stelio Pacca Loureiro Luna
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/12358-3 - Statistical improvement of the behavioral diagnosis of pain in felines, sheep, swine, and cattle
• Grantee: Pedro Henrique Esteves Trindade
• Support Opportunities: Scholarships in Brazil - Post-Doctoral