Changes in blood parameters and metabolism in bullfrog tadpoles, Lithobates catesbeianus, (Shaw, 1802) after exposure to the Sorocaba River (Sao Paulo, Brazil) water

Author(s)

Utsunomiya, Heidi Samantha Moraes ; Ferraz, Joao Victor Cassiel ; Fujiwara, Gabriel Hiroshi ; Gutierres, Davi Marques ; Fernandes, Isabela Ferreira ; Valverde, Bruno Serra de Lacerda ; de Oliveira, Classius ; Franco-Belussi, Lilian ; Fernandes, Marisa Narciso ; Carvalho, Cleoni dos Santos

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. N/A, p. 13-pg., 2022-12-08.

Abstract

This study evaluated the genetic damage, oxidative stress, neurotoxicity, and energy metabolism in bullfrog tadpoles (Lithobates catesbeianus) exposed to water from two sites of the Sorocaba River, Ibiuna (PI), and Itupararanga reservoir (PIR), in summer and winter. After 96-h exposure, the erythrocyte number decreased in PI and increase in PIR in summer. Bullfrogs show oxidative unbalance (liver, kidney, and muscle), with alterations in the nitric oxide synthase and glucose 6-phosphate dehydrogenase. Cholinesterase increased in the brain in PI and PIR in the summer and decreased in PI in the winter. It also increased in the muscle in both PI and PIR in the winter. Tadpoles show alterations in the activity of the metabolic enzymes (liver, kidney, and muscle), such as phosphofructokinase, pyruvatokinase, malate dehydrogenase, and lactate dehydrogenase; and in the amount of glucose and triglycerides metabolites. Exposure to the Sorocaba River reflected a stressful situation for L. catesbeianus as the changes caused to their metabolism associated with oxidative stress and neurotoxicity may have effects on the development of tadpoles. (AU)

• FAPESP's process: 20/06879-8 - Ecotoxicological evaluation of potentially toxic metals of the waters and the soil of the Pirjibú River by analysis of biochemical biomarkers in bullfrog tadpoles
• Grantee: João Victor Cassiel Ferraz
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/23781-9 - Ecotoxicological evaluation of water from the Sorocaba river using an integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802).
• Grantee: cleoni dos santos carvalho
• Support Opportunities: Regular Research Grants