Micro-vibration results in vitro-derived bovine blastocysts with greater cryotolerance, epigenetic abnormalities, and a massive transcriptional change

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dos Santos, Angelica C. ; Joaquim, Daniel C. ; Nociti, Ricardo P. ; Macabelli, Carolina H. ; Sampaio, Rafael V. ; Oliveira, Aline S. ; Pita, Maico O. ; de Oliveira, Robinson A. M. ; da Silveira, Juliano C. ; Meirelles, Flavio V. ; Watanabe, Osnir Y. ; Watanabe, Yeda F. ; Chiaratti, Marcos R.

• Document type: Journal article
• Source: Theriogenology; v. 196, p. 13-pg., 2023-01-15.

Abstract

Much effort has been employed to improve the quality of embryos obtained by in vitro production (IVP) given the relevance of this technology to current livestock systems. In this context, dynamic IVP systems have proved beneficial to the embryo once they mimic fluid flows and mechanical forces resulting from the movement of ciliated cells and muscle contraction in the reproductive tract. In the present study, we sought to confirm these initial findings as well as assess potential molecular consequences to the embryo by applying micro-vibration (45 Hz for 5 s once per 60 min) during both oocyte maturation and embryo culture in cattle. As a result, micro-vibration led to lower incidence of apoptosis in blastocysts following vitrification-thawing. Further analyses revealed epigenetic and transcriptional changes in blastocysts derived from the micro-vibration treatment, with a total of 502 differentially expressed genes. Enrich-ment analyses linked differentially expressed genes to 'Oxidative phosphorylation', 'Cytokine-cytokine receptor interaction', and 'Signaling pathways regulating pluripotency of stem cells'. Yet, a meta-analysis indicated that the transcriptional changes induced by micro-vibration were not toward that of in vivo -derived embryos. In conclusion, micro-vibration increases the cryoresistance of bovine embryos, but caution should be taken given the unclear consequences of epigenetic and transcriptional abnormalities induced by the treatment.(c) 2022 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 17/04372-0 - Mitochondrial DNA: mechanisms for genome integrity maintenance and impact on disease
• Grantee: Nadja Cristhina de Souza Pinto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/50231-6 - Molecular basis of mitochondrial inheritance: the role of mitochondrial fusion
• Grantee: Marcos Roberto Chiaratti
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 20/15412-6 - Role of mitochondria-endoplasmic reticulum interaction on maternal inheritance of cardiometabolic syndromes
• Grantee: Marcos Roberto Chiaratti
• Support Opportunities: Regular Research Grants