| Full text | |
| Author(s): Show less - |
Correia, Lucas Ian Veloso
;
de Vasconcelos Azevedo, Fernanda Van Petten
;
Amorim, Fernanda Gobbi
;
Cirilo Gimenes, Sarah Natalie
;
Polloni, Lorena
;
Pereira Zoia, Mariana Alves
;
Costa, Monica Soares
;
Rodrigues, Jessica Peixoto
;
Geraldo Yoneyama, Kelly A.
;
Santos, Jean Carlos
;
Arantes, Eliane Candiani
;
Rodrigues, Veridiana de Melo
;
Goulart, Luiz Ricardo
;
Rodrigues, Renata Santos
Total Authors: 14
|
| Document type: | Journal article |
| Source: | TOXINS; v. 14, n. 1, p. 22-pg., 2022-01-01. |
| Abstract | |
Some species of primitive predatory ants, despite living in a colony, exercise their hunting collection strategy individually; their venom is painful, paralyzing, digestive, and lethal for their prey, yet the toxins responsible for these effects are poorly known. Ectatomma opaciventre is a previously unrecorded solitary hunting ant from the Brazilian Cerrado. To overcome this hindrance, the present study performed the in vitro enzymatic, biochemical, and biological activities of E. opaciventre to better understand the properties of this venom. Its venom showed several proteins with masses ranging from 1-116 kDa, highlighting the complexity of this venom. Compounds with high enzymatic activity were described, elucidating different enzyme classes present in the venom, with the presence of the first L-amino acid oxidase in Hymenoptera venoms being reported. Its crude venom contributes to a state of blood incoagulability, acting on primary hemostasis, inhibiting collagen-induced platelet aggregation, and operating on the fibrinolysis of loose red clots. Furthermore, the E. opaciventre venom preferentially induced cytotoxic effects on lung cancer cell lines and three different species of Leishmania. These data shed a comprehensive portrait of enzymatic components, biochemical and biological effects in vitro, opening perspectives for bio-pharmacological application of E. opaciventre venom molecules. (AU) | |
| FAPESP's process: | 11/12317-3 - Cloning and heterologous expression of hyaluronidase and/or novel toxins obtained from the transcriptome of Tityus serrulatus' venom gland |
| Grantee: | Fernanda Gobbi Amorim |
| Support Opportunities: | Scholarships in Brazil - Doctorate |