Effects of Immunization with Recombinant Schistosoma mansoni Enzymes AK and HGPRT: Murine Infection Control

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Fattori, Ana Carolina Maragno ; Montija, Elisandra de A. ; Fragelli, Bruna D. de L. ; Correia, Ricardo de O. ; de Castro, Cynthia Aparecida ; Romanello, Larissa ; Nogueira, Camila T. T. ; Allegretti, Silmara M. M. ; Soares, Edson G. G. ; Pereira, Humberto D. D. ; Anibal, Fernanda de F.

• Document type: Journal article
• Source: PATHOGENS; v. 12, n. 1, p. 16-pg., 2023-01-01.

Abstract

Schistosomiasis is one of the most important human helminthiases worldwide. Praziquantel is the current treatment, and no vaccine is available until the present. Thus, the presented study aimed to evaluate the immunization effects with recombinant Schistosoma mansoni enzymes: Adenosine Kinase (AK) and Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT), as well as a MIX of the two enzymes. Female Balb/c mice were immunized in three doses, and 15 days after the last immunization, animals were infected with S. mansoni. Our results showed that the group MIX presented a reduction in the eggs in feces by 30.74% and 29%, respectively, in the adult worms. The groups AK, HGPRT and MIX could produce IgG1 antibodies, and the groups AK and MIX produced IgE antibodies anti-enzymes and anti-S. mansoni total proteins. The groups AK, HGPRT and MIX induced a reduction in the eosinophils in the peritoneal cavity. Besides, the group AK showed a decrease in the number of hepatic granulomas (41.81%) and the eggs present in the liver (42.30%). Therefore, it suggests that immunization with these enzymes can contribute to schistosomiasis control, as well as help to modulate experimental infection inducing a reduction of physiopathology in the disease. (AU)

• FAPESP's process: 14/07331-5 - Proteomics and bioprospecting of the effects of Mentha piperita L. during Schistosoma mansoni infection experimental model
• Grantee: Fernanda de Freitas Anibal
• Support Opportunities: Regular Research Grants