Schistosoma mansoni, parasite responsible for schistosomiasis, a disease that affects about 300 million people worldwide, lacks the synthetic pathway of purines, depending entirely on the way to salvation for their supply of purine bases of these. The enzyme adenosine kinase (AK), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), adenilosuccinato synthase (ADSS) and adenilosuccinato lyase (ADSL) are enzymes of this pathway. AK is directly responsible for phosphorylation of adenosine to adenosine monophosphate (AMP), HGPRT catalyzes the phosphorybosilation reversible guanine and hypoxanthine to IMP or GMP, ADSS catalyzes the conversion of IMP combined with aspartate at adenilsuccinato, ADSL hydrolyzed in adenilsuccinato fumarate and AMP . This route has been cited as a potential target for drug development against schistosomiasis. The main objectives of this project are: amplification and cDNA cloning, expression, purification and biochemical characterization of enzymes, to determine the catalytic constants for the various enzyme substrates and three-dimensional structure by X-ray diffraction. This work is part of a larger project aimed at getting all the structures of enzymes involved in purine pathway of salvation of Schistosoma mansoni.
News published in Agência FAPESP Newsletter about the scholarship: