| Full text | |
| Author(s): |
Frezarim, Gabriela B.
;
Fonseca, Larissa F. S.
;
Salatta, Bruna M.
;
Silva, Danielly B. S.
;
Bresolin, Tiago
;
Seno, Leonardo de Oliveira
;
Barufatti, Alexeia
;
Ferro, Jesus Aparecido
;
Albuquerque, Lucia Galvao
Total Authors: 9
|
| Document type: | Journal article |
| Source: | GENOME; v. 65, n. 4, p. 12-pg., 2022-04-01. |
| Abstract | |
Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real- time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle. (AU) | |
| FAPESP's process: | 18/20026-8 - Multi-user equipment approved in grant 2017/10630-2: server |
| Grantee: | Lucia Galvão de Albuquerque |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 17/10630-2 - Genetic aspects of meat production quality, efficiency and sustainability in Nelore breed animals |
| Grantee: | Lucia Galvão de Albuquerque |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/23937-6 - Expression of genes and proteins related to meet quality in Nelore cattle |
| Grantee: | Lucia Galvão de Albuquerque |
| Support Opportunities: | Regular Research Grants |