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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Feline leishmaniosis: hematological and biochemical analysis

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Author(s):
Diogo Tiago da Silva ; Maria Luana Alves ; Júlio Cesar Pereira Spada ; João Augusto Franco Leonel ; Geovanna Vioti [5] ; Julia Cristina Benassi [6] ; Valéria Maria Lara Carregaro [7] ; Maria Fernanda Alves-Martin [8] ; Wilma Aparecida Starke-Buzetti [9] ; Trícia Maria Ferreira de Sousa Oliveira
Total Authors: 10
Document type: Journal article
Source: Revista Brasileira de Parasitologia Veterinária; v. 32, n. 2 2023-06-26.
Abstract

Abstract One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) and parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) and 1.8% (03/166) were positive by ELISA, IFAT, both PCRs and PA, respectively. The sequencing of ITS-1 PCR amplicons revealed a 100% match with Leishmania infantum. After the Leishmania spp. survey, 12 cats were selected and divided into two groups for clinical, hematological, and biochemical analysis: six L. infantum positive cats (G1) and six Leishmania spp. negative cats (G2). All the cats were negative for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). A statistical analysis indicated significantly low platelet counts and significant hyperproteinemia associated with hypoalbuminemia in positive cats (p<0.05). Our results suggest that in endemic areas, cats with clinical signs of feline leishmaniosis (such as skin lesions, weight loss and/or enlarged lymph nodes) and that exhibit hematological and biochemical changes, such as low platelet counts and hyperproteinemia with hypoalbuminemia, should be tested for Leishmania spp. infection. (AU)

FAPESP's process: 16/08018-4 - Humoral and cellular immune response of cats and horses infected by Leishmania spp.
Grantee:Trícia Maria Ferreira de Sousa Oliveira
Support Opportunities: Regular Research Grants