Time-restricted feeding combined with aerobic exercise training can prevent weight gain and improve metabolic disorders in mice fed a high-fat diet

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Vieira, Renan Fudoli Lins ; Munoz, Vitor Rosetto ; Junqueira, Rafael Lima ; de Oliveira, Fellipe ; Gaspar, Rafael Calais ; Nakandakari, Susana Castelo Branco Ramos ; Costa, Suleyma de Oliveira ; Torsoni, Marcio Alberto ; da Silva, Adelino S. R. ; Cintra, Dennys Esper ; de Moura, Leandro Pereira ; Ropelle, Eduardo Rochete ; Zaghloul, Iman ; Mekary, Rania A. ; Pauli, Jose Rodrigo

• Document type: Journal article
• Source: JOURNAL OF PHYSIOLOGY-LONDON; v. 600, n. 4, p. 17-pg., 2021-01-26.

Abstract

Time-restricted feeding (TRF) or physical exercise have been shown to be efficient in the prevention and treatment of metabolic disorders; however, the additive effects of TRF combined with aerobic exercise (AE) training on liver metabolism have not been widely explored. In this study TRF (8 h in the active phase) and TRF combined with AE (TRF+Exe) were compared in male Swiss mice fed a high-fat diet, with evaluation of the effects on insulin sensitivity and expression of hepatic genes involved in fatty acid oxidation, lipogenesis and gluconeogenesis. As in previous reports, we show that TRF alone (eating only between zeitgeber time 16 and 0) was sufficient to reduce weight and adiposity gain, increase fatty acid oxidation and decrease lipogenesis genes in the liver. In addition, we show that mice of the TRF+Exe group showed additional adaptations such as increased oxygen consumption ((V) over dotO(2)), carbon dioxide production ((V) over dot(CO2)) and production of ketone bodies (beta-hydroxybutyrate). Also, TRF+Exe attenuated the negative effects of high-fat diet feeding on the insulin signalling pathway (insulin receptor, insulin receptor substrate, Akt), and led to increased fatty acid oxidation (Ppara, Cpt1a) and decreased gluconeogenic (Fbp1, Pck1, Pgc1a) and lipogenic (Srebp1c, Cd36) gene expression in the liver. These molecular results were accompanied by increased glucose metabolism, lower serum triglycerides and reduced hepatic lipid content in the TRF+Exe group. The data presented in this study show that TRF alone has benefits but TRF+Exe has additive benefits and can mitigate the harmful effects of consuming a high-fat diet on body adiposity, liver metabolism and glycaemic homeostasis in young male Swiss mice. (AU)

• FAPESP's process: 19/00227-1 - Effects mediated by the exercise and metformin on rock protein and the regulation of lipogenesis and glucose production in the liver of rodents with celiac disease
• Grantee: José Rodrigo Pauli
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 18/20872-6 - Effects of physical exercise on Rock protein signaling pathway, and lipogenic regulation and gluconeogenesis in the liver of aged rats
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/21709-4 - Implications of mitonuclear imbalance and UPRmt in hypothalamic neurons in the genesis of Obesity
• Grantee: Eduardo Rochete Ropelle
• Support Opportunities: Research Projects - Thematic Grants