Amperometric detection of indapamide on glassy carbon electrode

Author(s)

Oliveira, Thawan G. ; Santos, Berlane G. ; Goncalves, Josue M. ; Angnes, Lucio

• Document type: Journal article
• Source: Electroanalysis; v. 35, n. 9, p. 9-pg., 2023-05-11.

Abstract

This work describes the development of a fast, precise and reliable voltammetric method for the quantification of indapamide, an orally active diuretic sulfonamide used for hypertensive treatment. This compound acts inhibiting sodium reabsorption and increasing the elimination of water. This characteristic was responsible for its banishment by the International Olympic Committee since 1999. The study begins by finding an adequate potential range (-0.20 to 0.30 V) to avoid poisoning the working glassy carbon electrode (GCE) in phosphate buffer 0.10 mol L-1 (pH=12.0). Utilizing flow injection analysis, linear responses between 2.0 x 10(-6) mol L-1 to 2.5 x 10(-5) mol L-1 of indapamide (R-2=0.995), and detection limit (LOD) 3.0 x 10(-7) mol L-1 were obtained. This method was applied for the quantification of indapamide in tablets and in synthetic urine. The same flow system was used for the analysis of commercial drugs and the response obtained corresponded to 98 % of the concentration indicated on the drug label. These tablets were also analyzed by high performance liquid chromatography (HPLC), obtaining a recovery of 103 % and LOD 4.0 x 10(-7) mol L-1. The velocity of analysis using flow methods compares advantageously to the classical chromatographic methods. For synthetic urine, linear responses were obtained in samples spiked in the region from 5.0 x 10(-6) mol L-1 to 30 x 10(-6) mol L-1 (R-2=0.991) and LOD 3.0 x 10(-7) mol L-1. (AU)

• FAPESP's process: 18/16896-7 - Development of amperometric and impedimetric sensors based on double hydroxides of nickel and vanadium (alpha-NixV1-x(OH)2) and their nanocomposites with expandable graphite/graphene
• Grantee: Josué Martins Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants