Cervical carcinoma induces NLRP3 inflammasome activation and IL-1ss release in human peripheral blood monocytes affecting patients' overall survival

Author(s)

Fernandes, Fernanda Pereira ; Cambui, Raylane Adrielle Goncalves ; Soares, Jaine Lima da Silva ; Reis, Edione Cristina dos ; Leal, Vinicius Nunes Cordeiro ; Pontillo, Alessandra

• Document type: Journal article
• Source: Clinical & Translational Oncology; v. 25, n. 11, p. 10-pg., 2023-06-16.

Abstract

PurposeOur group previously demonstrated that genetic variants in inflammasome genes contribute to protection against the establishment of human papilloma virus (HPV)-associated cervical carcinoma (CC). The objective of this study was to better understand the contribution of inflammasome and its cytokines in the CC microenvironment.MethodsThe inflammasome activation was analyzed in CC tumoral cell lines and healthy donors (HD)' monocytes in co-culture. In vitro results were then compared to CC patients' public databases.ResultsCC cells did not produce IL-1ss or IL-18 themselves, however, when in co-culture with HD monocytes, induced IL-1ss release in those leucocytes. Inflammasome activation appears to be partially dependent on the NLRP3 receptor. Public data analysis revealed that IL1B expression is increased in the CC compared to normal uterine cervix, and that patients with high IL1B expression had a shorter overall survival.ConclusionCC microenvironment can activate the inflammasome and IL-1ss release in surrounding monocytes, which could be detrimental for CC prognosis. (AU)

• FAPESP's process: 17/10824-1 - Genetic and functional characterization of NLRP-3 in lymphocytes and monocytes from chronically infected HIV-1 individuals
• Grantee: Vinícius Nunes Cordeiro Leal
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/06363-4 - Modulator effect of TORID on the pathological activation of inflammasome: a genetic-based approach
• Grantee: Alessandra Pontillo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/50650-7 - Characterization of novel molecular players in the control of obesity and obesity-induced inflammation
• Grantee: Alessandra Pontillo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/04361-1 - Evaluation of the activation of inflammasome in the context of HPV and cervical cancer associated with HPV
• Grantee: Fernanda Pereira Fernandes
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/17373-0 - Genetic and functional characterization of dendritic cells of HIV + patients stimulated with flagellin: implementation of immunotherapy against HIV-1
• Grantee: Edione Cristina dos Reis
• Support Opportunities: Scholarships in Brazil - Doctorate