Oxidative Machinery of basidiomycetes as potential enhancers in lignocellulosic biorefineries: A lytic polysaccharide monooxygenases approach

Author(s)

Barrios-Gutierrez, Solange Grace ; Velez-Mercado, Martha Ines ; Ortega, Julia Rodrigues ; Lima, Awana da Silva ; Saraiva, Ana Luiza da Rocha Fortes ; Berto, Gabriela Leila ; Segato, Fernando

• Document type: Journal article
• Source: Bioresource Technology; v. 386, p. 11-pg., 2023-10-01.

Abstract

Basidiomycetes are renowned as highly effective decomposers of plant materials, due to their extensive array of oxidative enzymes, which enable them to efficiently break down complex lignocellulosic biomass structures. Among the oxidative machinery of industrially relevant basidiomycetes, the role of lytic polysaccharide monooxygenases (LPMO) in lignocellulosic biomass deconstruction is highlighted. So far, only a limited number of basidiomycetes LPMOs have been identified and heterologously expressed. These LPMOs have presented activity on cellulose and hemicellulose, as well as participation in the deconstruction of lignin. Expanding on this, the current review proposes both enzymatic and non-enzymatic mechanisms of LPMOs for biomass conversion, considering the significance of the Carbohydrate-Binding Modules and other C-terminal regions domains associated with their structure, which is involved in the deconstruction of lignocellulosic biomass. (AU)

• FAPESP's process: 22/00539-6 - Computational analysis and synthetic biology approaches to understand the interaction and electron transfer between CDH and LPMO
• Grantee: Fernando Segato
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/04227-9 - Multiuser equipment granted in the proposal 2021/06679-1: Dionex ICS600 Capillary HPIC system
• Grantee: Fernando Segato
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 22/04713-0 - Exploring the interaction between lytic polysaccharide monooxygenases and its redox partner cellobiose dehydrogenases
• Grantee: Martha Inés Vélez Mercado
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/06679-1 - Enzymatic oxidation of sugarcane bagasse 2: exploring the interaction between LPMOs and its redox partner CDHs aiming for the development of more efficient enzymes for insertion into an engineered cell factory
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants - Phase 2