| Full text | |
| Author(s): |
Csillag, Kinga
;
Emri, Tamas
;
Rangel, Drauzio E. N.
;
Pocsi, Istvan
Total Authors: 4
|
| Document type: | Journal article |
| Source: | FUNGAL BIOLOGY; v. 127, n. 7-8, p. 7-pg., 2023-07-01. |
| Abstract | |
The azo dye Congo Red (CR) is frequently used as an agent to elicit cell wall integrity stress in fungi. This highly toxic aromatic, heterocyclic compound contains two azo bonds as chromophore, which are responsible for protonation under acidic conditions, leading to changes in the molecular structure of the dye and the color of the solution. The investigation of how CR affects the growth of Aspergillus nidulans and Aspergillus niger on surface cultures provided us with evidence about its pH-dependent toxicity. Reducing the starting pH of the media from 7 to 3 decreased both the toxicity of CR and the dosedependence of its toxicity substantially. These changes can be explained by the pH-dependent structural changes of CR and its precipitation at low pH. The pH also depended on the fungi; they could induce a decrease or even an increase, which could be important in the loss of dose-dependence. Our experiments led to the conclusion that in studies to evaluate the antifungal effect of CR, properly buffered solutions with pH values adjusted to above 5 are highly recommended to achieve a well-detectable and dose-dependent antifungal effect. However, for decolorization of CR solutions, lower pH is suggested where the decreased toxicity and solubility of CR could help this process.& COPY; 2022 The Authors. Published by Elsevier Ltd on behalf of British Mycological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). (AU) | |
| FAPESP's process: | 21/13614-3 - IV International Symposium on Fungal Stress - ISFUS & XIII International Fungal Biology Conference - IFBC |
| Grantee: | Drauzio Eduardo Naretto Rangel |
| Support Opportunities: | Organization Grants - Scientific Meeting |
| FAPESP's process: | 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions |
| Grantee: | Drauzio Eduardo Naretto Rangel |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi |
| Grantee: | Drauzio Eduardo Naretto Rangel |
| Support Opportunities: | Research Grants - Young Investigators Grants |