Cytotoxic activity of Ru(II)/DPEPhos/N,S-mercapto complexes (DPEPhos = bis-[(2-diphenylphosphino)phenyl]ether)

Author(s):Show less -

Grawe, Gregory F. ; Oliveira, Katia M. ; Leite, Celisnolia M. ; de Oliveira, Tamires D. ; Costa, Analu R. ; Moraes, Carlos A. F. ; Honorato, Joao ; Cominetti, Marcia R. ; Castellano, Eduardo E. ; Correa, Rodrigo S. ; Machado, Sergio P. ; Batista, Alzir A.

• Document type: Journal article
• Source: Journal of Inorganic Biochemistry; v. 244, p. 11-pg., 2023-07-01.

Abstract

We report here on three new ruthenium(II) complexes, [Ru(DPEPhos)(mtz)(bipy)]PF6 (Ru1), [Ru(DPEPhos) (mmi)(bipy)]PF6 (Ru2) and [Ru(DPEPhos)(dmp)(bipy)]PF6 (Ru3). DPEPhos = bis-[(2-diphenylphosphino) phenyl]ether, mtz = 2-mercapto-2-thiazoline, mmi = 2-mercapto-1-methylimidazole, dmp = 4,6-diamino-2mercaptopyrimidine and bipy = 2,2 & PRIME;-bipyridine. The compounds were characterized by several spectroscopic techniques, and the molecular structure of Ru1 complex was determined by single-crystal X-ray diffraction. The cytotoxicity of Ru1 - Ru3 complexes were tested against the A549 (human lung) and the MDA-MB-231 (human breast) cancer cell lines and against MRC-5 (non-tumor lung) and MCF-10A (non-tumor breast) cell lines through the MTT assay. All three complexes are cytotoxic against the cell lines studied, with IC50 values lower than those found for the cisplatin. Among them, the Ru2 complex has shown the best selectivity against MDA-MB-231 cancer cell lines, with an IC50 value 12 times lower than that on MCF-10A. The complex Ru2 was capable to induce changes in MDA-MB-231 cells morphology, with loss of cellular adhesion, inhibited colony formation and induce an accumulation of cells at the sub-G1 phase, with an increase in S-phase and decrease of cells at G2 phase. Viscosity, electrochemical and Hoechst 33258 displacement experiments for Ru1 - Ru3 complexes with calf thymus DNA (CT-DNA) showed an electrostatic and groove binding mode of interaction. Additionally, the complexes interact with the protein Human Serum Albumin (HSA) by static mechanism. The negative values for & UDelta;H and & UDelta;S indicate that van der Waals forces and hydrogen bonding may occurs between the complexes and HSA. Therefore, this class of complexes are promising anticancer candidates and may be selected to further detailed studies. (AU)

• FAPESP's process: 21/14210-3 - Investigation of the effectiveness of ruthenium complexes in the treatment of cancer: in vitro and in vivo studies
• Grantee: Katia Mara de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/15850-0 - X-ray diffraction as a tool in potential drug development
• Grantee: Eduardo Ernesto Castellano
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/14561-8 - Study in vitro and in vivo of Ru(II) phosphine complexes with anticancer activities
• Grantee: Alzir Azevedo Batista
• Support Opportunities: Regular Research Grants