| Full text | |
| Author(s): |
Bueno, Manuela Rocha
;
Dudu-Silva, Gustavo
;
Macedo, Tatiane Tiemi
;
Gomes, Aline Paim de Abreu Paulo
;
Braga, Arthur Rodrigues Oliveira
;
Aguiar Silva, Lucas Daylor
;
Bueno-Silva, Bruno
Total Authors: 7
|
| Document type: | Journal article |
| Source: | FRONTIERS IN DENTAL MEDICINE; v. 4, p. 7-pg., 2023-07-31. |
| Abstract | |
The present study evaluated the antibiofilm effects of Lactobacillus acidophilus within a subgingival multispecies biofilm. Lactobacillus acidophilus (La5) at 1 x 10(2), 1 x 10(4), and 1 x 10(6) were included at the beginning of biofilm formation, which lasted 7 days. The biofilms comprised 33 periodontitis-related bacterial species and the Calgary Biofilm device was used. At the end, DNA-DNA hybridization (checkerboard) was performed. A Kruskal-Wallis test followed by a Dunn post hoc test were performed (p & LE; 0.05). La5 at 1 x 10(4) and 1 x 10(6) reduced the total counts of biofilm and the proportions of red and green complexes when compared to the control biofilm without La5 (p & LE; 0.05). La5 at 1 x 10(4) increased the proportions of Actinomyces complex compared to the controls (p & LE; 0.05). Both La5 at 1 x 10(4) and 1 x 10(6) decreased levels of 20 and 14 distinct species, respectively, including Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum polymorphum, and Parvimonas micra compared to the control (p & LE; 0.05). Only La5 at 1 x 10(4) reduced the levels of Tannerella forsythia, Fusobacterium periodonticum, and Aggregatibacter actinomycetencomytans compared to the control (p & LE; 0.05). L. acidophilus inhibited establishing periodontic pathogens from red complex such as P. gingivalis and T. forsythia in a subgingival multispecies biofilm. (AU) | |
| FAPESP's process: | 17/16377-7 - EVALUATION OF THE EFFECT OF PROBIOTIC BACTERIA ON THE IMUNE RESPONSE TO A.ACTINOMYCETEMCOMITANS: A STUDY IN GINGIVAL EPITHELIAL CELLS AND IN EXPERIMENTAL MODEL OF INFECTION IN VIVO. |
| Grantee: | Manuela Rocha Bueno |
| Support Opportunities: | Scholarships in Brazil - Doctorate |