| Full text | |
| Author(s): Show less - |
Negahdary, Masoud
;
Veloso, William Barros
;
Bacil, Raphael Prata
;
Buoro, Rafael Martos
;
Gutz, Ivano Gebhardt Rolf
;
Paixao, Thiago Regis Longo Cesar
;
do Lago, Claudimir Lucio
;
Sakata, Solange Kazumi
;
Meloni, Gabriel Negrao
;
Franca, Mesaque Carvalho
;
de Oliveira, Thawan Gomes
;
Ameku, Wilson Akira
;
Durazzo, Michelangelo
;
Angnes, Lucio
Total Authors: 14
|
| Document type: | Journal article |
| Source: | SENSORS AND ACTUATORS B-CHEMICAL; v. 393, p. 11-pg., 2023-10-15. |
| Abstract | |
In this study, beta-amyloid (A beta((1-42))), an essential biomarker for diagnosing Alzheimer's disease (AD), was detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: similar to 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times, compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created an optimized biointerface to detect A beta((1-42)) in a linear range from 0.1 pg mL(-1) to 10 ng mL(-1) (limit of detection (LOD): 84 fg mL(-1), (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and human serum. (AU) | |
| FAPESP's process: | 19/27021-4 - Design and fabrication of electrochemical biosensors for early diagnosis of myocardial infarction and Hepatocellular Carcinoma using microRNAs (miRNAs) primers as biorecognition element and arrays of gold nanostructures |
| Grantee: | Masoud Negahdary |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium |
| Grantee: | Claudimir Lucio Do Lago |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications |
| Grantee: | Mauro Bertotti |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |