| Full text | |
| Author(s): |
dos Anjos, Eli Heber Martins
;
Rocha, Marina Amorim
;
de Campos Vidal, Benedicto
;
Mello, Maria Luiza Silveira
Total Authors: 4
|
| Document type: | Journal article |
| Source: | CYTOLOGIA; v. 88, n. 3, p. 6-pg., 2023-09-01. |
| Abstract | |
Sodium valproate (VPA) is an epidrug that inhibits histone deacetylases and favors histone acetylation and DNA demethylation, thereby inducing chromatin remodeling in several cell types. Although VPA induced heterochromatin decondensation in the hemipteran Triatoma infestans, this phenomenon was not favored by histone acetylation. Considering that DNA demethylation leading to chromatin unpacking is another consequence of VPA action in several organisms, we used immunofluorescence to investigate whether cytosine methylation was the target of VPA action that affected heterochromatin remodeling in T. infestans. In addition, Fourier transform infrared (FTIR) analysis was used to evaluate the participation of cytosine in T. infestans DNA. Immunofluorescence results indicated the absence of 5-methylcytosine signals in heterochromatin, but the presence of these signals in euchromatin, where demethylation was promoted by VPA. The FTIR spectra of T. infestans global DNA exhibited marks indicative of AT richness and a low representation of cytosine contribution. In conclusion, the chromatin remodeling event previously reported in the heterochromatin compartment of T. infestans in response to VPA, which was not due to histone acetylation, was also not due to DNA cytosine demethylation. (AU) | |
| FAPESP's process: | 15/10356-2 - Action of valproic acid on chromatin structure and function |
| Grantee: | Maria Luiza Silveira Mello |
| Support Opportunities: | Research Projects - Thematic Grants |