Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects

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Buss, Leonardo Fernandes ; de Martin, Gustavo Sigrist ; Martinez, Elizabeth Ferreira ; Filgueiras, Isabela Amanda de Abreu Araujo Porcaro ; Magnabosco, Jose Luiz ; Alves, Bruno Frenhan ; Almeida, Bruno de Macedo ; Kotaka, Tatiana ; Teixeira, Marcelo Lucchesi ; Ferreira, Jose Ricardo Muniz ; da Rocha, Daniel Navarro ; Canal, Raul ; Aloise, Antonio Carlos ; Holliday, Lexie Shannon ; Pelegrine, Andre Antonio

• Document type: Journal article
• Source: JOURNAL OF FUNCTIONAL BIOMATERIALS; v. 14, n. 8, p. 12-pg., 2023-08-01.

Abstract

The aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was frozen and lyophilized. After general anesthesia, an 8 mm diameter bone defect was created in the calvaria of twenty-four rats. The defects were filled with the following materials: xenograft alone (G1) or xenograft associated with lyophilized conditioned medium (G2). After 14 or 42 days, the animals were euthanized, and the specimens processed for histologic and immunohistochemical analysis. Bone formation at the center of the defect was observed only in the G2 at 42 days. At both timepoints, increased staining for VEGF, a marker for angiogenesis, was observed in G2. Consistent with this, at 14 days, G2 also had a higher number of blood vessels detected by immunostaining with an anti-CD34 antibody. In conclusion, conditioned media from human dental pulp mesenchymal stem cell cultures had a positive effect on the regenerative process in rat critical-size bone defects. Both the formation of bone and enhancement of vascularization were stimulated by the conditioned media. (AU)

• FAPESP's process: 18/18890-6 - Development of biofunctional materials for application in regenerative medicine, tissue engineering and advanced therapy of bone defects
• Grantee: Daniel Navarro da Rocha
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• FAPESP's process: 17/08198-5 - Development of composites membranes for guided bone regeneration (GBR)
• Grantee: Daniel Navarro da Rocha
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE