| Full text | |
| Author(s): |
Klafke, Karina
;
Sanches, Marcio Martinello
;
Sihler, William
;
de Souza, Marlinda Lobo
;
Tonso, Aldo
Total Authors: 5
|
| Document type: | Journal article |
| Source: | PATHOGENS; v. 12, n. 8, p. 13-pg., 2023-08-01. |
| Abstract | |
Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10(11) OBs/L. (AU) | |
| FAPESP's process: | 17/06371-1 - Development of production process of baculovirus biopesticide by Sf9 insect cells in bioreactor |
| Grantee: | Karina Klafke |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/08526-5 - Baculovirus production comparing STR and wave bioreactors |
| Grantee: | Karina Klafke |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |