| Full text | |
| Author(s): |
Metcalfe, Cushla J.
;
Oliveira, Sarah G.
;
Gaiarsa, Jonas W.
;
Aitken, Karen S.
;
Carneiro, Monalisa S.
;
Zatti, Fernanda
;
Van Sluys, Marie-Anne
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Journal of Experimental Botany; v. 66, n. 14, p. 12-pg., 2015-07-01. |
| Abstract | |
Sugarcane is the main source of the world's sugar and is becoming increasingly important as a source of biofuel. The highly polyploid and heterozygous nature of the sugarcane genome has meant that characterization of the genome has lagged behind that of other important crops. Here we developed a method using a combination of quantitative PCR with a transposable marker system to score the relative number of alleles with a transposable element (TE) present at a particular locus. We screened two genera closely related to Saccharum (Miscanthus and Erianthus), wild Saccharum, traditional cultivars, and 127 modern cultivars from Brazilian and Australian breeding programmes. We showed how this method could be used in various ways. First, we showed that the method could be extended to be used as part of a genotyping system. Secondly, the history of insertion and timing of the three TEs examined supports our current understanding of the evolution of the Saccharum complex. Thirdly, all three TEs were found in only one of the two main lineages leading to the modern sugarcane cultivars and are therefore the first TEs identified that could potentially be used as markers for Saccharum spontaneum. (AU) | |
| FAPESP's process: | 08/52074-0 - Sugarcane genome sequence: plant transposable elements are active contributors to gene structure variation, regulation and function |
| Grantee: | Marie-Anne Van Sluys |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 13/09391-2 - Using Real-Time PCR to quantify retrotransposon-based insertion polymorphism in the modern polyploid sugarcane cultivars |
| Grantee: | Cushla J Metcalfe |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |