| Full text | |
| Author(s): Show less - |
da Costa, Hernan H. M.
;
Orts, Diego J. B.
;
Moura, Andrew D.
;
Duarte-Neto, Amaro N.
;
Cirqueira, Cinthya S.
;
Ressio, Rodrigo A.
;
Kanamura, Cristina T.
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Miguita, Karen
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Ferreira, Jerenice E.
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Santos, Raimunda T. M.
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Adriani, Patricia P.
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Cunha-Junior, Jair P.
;
Astray, Renato M.
;
Catarino, Regina M.
;
Lancelotti, Marcelo
;
Prudencio, Carlos R.
Total Authors: 16
|
| Document type: | Journal article |
| Source: | Viruses-Basel; v. 15, n. 2, p. 16-pg., 2023-02-01. |
| Abstract | |
Neutralizing antibodies (nAbs) are a critical part of coronavirus disease 2019 (COVID-19) research as they are used to gain insight into the immune response to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infections. Among the technologies available for generating nAbs, DNA-based immunization methods are an alternative to conventional protocols. In this pilot study, we investigated whether DNA-based immunization by needle injection in rabbits was a viable approach to produce a functional antibody response. We demonstrated that three doses of DNA plasmid carrying the gene encoding the full-length spike protein (S) or the receptor binding domain (RBD) of SARS-CoV-2 induced a time-dependent increase in IgG antibody avidity maturation. Moreover, the IgG antibodies displayed high cross neutralization by live SARS-CoV-2 and pseudoviruses neutralization assays. Thus, we established a simple, low cost and feasible DNA-based immunization protocol in rabbits that elicited high IgG avidity maturation and nAbs production against SARS-CoV-2, highlighting the importance of DNA-based platforms for developing new immunization strategies against SARS-CoV-2 and future emerging epidemics. (AU) | |
| FAPESP's process: | 17/50333-7 - Institutional research development plan of the Instituto Adolfo Lutz (PDIp) |
| Grantee: | Carlos Henrique Camargo |
| Support Opportunities: | Research Grants - State Research Institutes Modernization Program |