| Full text | |
| Author(s): Show less - |
Trinca, Vitor
;
Carli, Sibeli
;
Uliana, Joao Vitor Cardoso
;
Garbelotti, Carolina Victal
;
da Silva, Mariana Mendes
;
Kunes, Vitor
;
Meleiro, Luana Parras
;
Brancini, Guilherme Thomaz Pereira
;
Menzel, Frank
;
Andrioli, Luiz Paulo Moura
;
Torres, Tatiana Teixeira
;
Ward, Richard John
;
Monesi, Nadia
Total Authors: 13
|
| Document type: | Journal article |
| Source: | ISCIENCE; v. 26, n. 4, p. 27-pg., 2023-04-21. |
| Abstract | |
Soil biota has a crucial impact on soil ecology, global climate changes, and effective crop management and studying the diverse ecological roles of dipteran larvae deepens the understanding of soil food webs. A multi-omics study of Pseudolycoriella hygida comb. nov. (Diptera: Sciaroidea: Sciaridae) aimed to characterize carbohydrate-active enzymes (CAZymes) for litter degradation in this species. Manual curation of 17,881 predicted proteins in the Psl. hygida genome identified 137 secreted CAZymes, of which 33 are present in the saliva proteome, and broadly confirmed by saliva CAZyme catalytic profiling against plant cell wall polysaccharides and pNP-glycosyl substrates. Comparisons with two other sciarid species and the outgroup Lucilia cuprina (Diptera: Calliphoridae) identified 42 CAZyme families defining a sciarid CAZyme profile. The litter-degrading potential of sciarids corroborates their significant role as decomposers, yields insights to the evolution of insect feeding habits, and highlights the importance of insects as a source of biotechnologically relevant enzymes. (AU) | |
| FAPESP's process: | 14/14318-5 - Gene interactions in the anterior region of the syncytial blastoderm of Drosophila melanogaster and of the sciarid fly Trichosia pubescens |
| Grantee: | Luiz Paulo Moura Andrioli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/05636-4 - Dimensions US-BIOTA-São Paulo: more to the blow fly than meets the eye: understanding evolutionary and genetic origins of diverse trophic specializations |
| Grantee: | Tatiana Teixeira Torres |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 16/17582-0 - Thermostability modulation of a ß-glucosidase stimulated by glucose and xylose using site directed glycosylation |
| Grantee: | Luana Parras Meleiro Garcia |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/24139-6 - Design and Characterization of Multifunctional Xylanolytic Enzymes |
| Grantee: | Richard John Ward |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/25325-8 - The dipteran protoracic glands: morphological, genomic and functional studies in the dipteran B. hygida and D. melanogaster |
| Grantee: | Nadia Monesi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/13734-3 - Engineering of an endopolygalacturonase by insertion of different Carbohydrate Binding Modules (CBMs): chimerogenesis and proximity effects |
| Grantee: | Sibeli de Carli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/25664-2 - Expression and purification of recombinants xylanolitics enzymes and study of their activities against sugar cane extracted arabinoxylans |
| Grantee: | Carolina Victal Garbelotti |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |