Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells

Author(s)

Pedrosa, Valber A. ; Chen, Kangfu ; George, Thomas J. ; Fan, Z. Hugh

• Document type: Journal article
• Source: BIOSENSORS-BASEL; v. 13, n. 7, p. 9-pg., 2023-07-01.

Abstract

Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% & PLUSMN; 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays. (AU)

• FAPESP's process: 19/13411-5 - Development of microfluidic device for liquid biopsy of circulating tumor cells
• Grantee: Valber de Albuquerque Pedrosa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/25937-6 - Development of microfluidic device for monitoring circulating tumor cells
• Grantee: Valber de Albuquerque Pedrosa
• Support Opportunities: Scholarships abroad - Research