Comparison of the Metabolic Profile between Unstimulated and Stimulated Saliva Samples from Pregnant Women with/without Obesity and Periodontitis

Author(s)

Foratori-Junior, Gerson Aparecido ; Le Guennec, Adrien ; Fidalgo, Tatiana Kelly da Silva ; Jarvis, James ; Mosquim, Victor ; Buzalaf, Marilia Afonso Rabelo ; Carpenter, Guy Howard ; Sales-Peres, Silvia Helena de Carvalho

• Document type: Journal article
• Source: JOURNAL OF PERSONALIZED MEDICINE; v. 13, n. 7, p. 21-pg., 2023-07-01.

Abstract

This study aimed to compare the metabolic profile of unstimulated (US) and stimulated (SS) saliva samples from pregnant women with/without obesity and periodontitis. Ninety-six pregnant women were divided into: obesity + periodontitis (OP = 20); obesity/no periodontitis (OWP = 27); normal BMI + periodontitis (NP = 20); and normal BMI/no periodontitis (NWP = 29). US and SS samples were collected by expectoration and chewing of sterilized parafilm gum, respectively, and samples were individually analyzed by Proton Nuclear Magnetic Resonance (H-1-NMR). Univariate (t test and correlations) and multivariate (Principal Component Analysis-PCA, and Partial Least Square-Discriminant Analysis-PLS-DA with Variance Importance Projection-VIP scores) and Metabolite Set Enrichment Analysis were done (p < 0.05). Metabolites commonly found in all groups in elevated concentration in US samples were 5-Aminopentoate, Acetic acid, Butyric acid, Propionic acid, Pyruvic acid, and Succinic acid. They were mainly related to the butyrate metabolism, citric acid cycle, amino sugar metabolism, fatty acids biosynthesis, pyruvate metabolism, glutamate metabolism, and Warburg effect. Metabolites commonly found in all groups that were in elevated concentration in SS samples were Citrulline, Fumaric acid, Histidine, N-acetyl glutamine, N-acetylneuraminic acid, para-hydroxyphenylacetic acid, Proline, Tyrosine. Although some differences were found between unstimulated and stimulated saliva samples from pregnant women with/without obesity and periodontitis, stimulated saliva collection seems adequate, demonstrating similar metabolic pathways to unstimulated saliva samples when groups are compared. (AU)

• FAPESP's process: 18/20626-5 - Evaluation of the proteins differentially expressed in saliva associated with periodontitis during the 3rd trimester of pregnancy in obese and eutrophic patients
• Grantee: Gerson Aparecido Foratori Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/26845-3 - Systemic and oral evaluations and metabolomic profiling of the saliva and plasma by quantitative proton nuclear magnetic resonance (1H-NMR) in obese and eutrophic pregnant women
• Grantee: Gerson Aparecido Foratori Junior
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate