Mining reactive triplet carbonyls in biological systems

Author(s)

Ramos, Luiz D. ; Gomes, Thiago M. V. ; V. Stevani, Cassius ; Bechara, Etelvino J. H.

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 243, p. 16-pg., 2023-06-01.

Abstract

Aliphatic triplet carbonyls can be treated as short-lived radicals, since both species share similar reactions such as hydrogen atom abstraction, cyclization, addition, and isomerization. Importantly, enzyme-generated triplet carbonyls excite triplet molecular oxygen to the highly reactive, electrophilic singlet state by resonance energy transfer, which can react with proteins, lipids, and DNA. Carbonyl triplets, singlet oxygen, and radicals are endowed with the potential to trigger both normal and pathological responses. In this paper, we present a short review of easy, fast, and inexpensive preliminary tests for the detection of transient triplet carbonyls in chemical and biological systems. This paper covers direct and indirect methods to look for triplet carbonyls based on their spectral distribution of chemiluminescence, photoproduct analysis, quenching of light emission by conjugated dienes, and enhancement of light emission by the sensitizer 9,10-dibromoanthracence-2-sulfonate ion (DBAS). (AU)

• FAPESP's process: 17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark
• Grantee: Etelvino José Henriques Bechara
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/03864-5 - Quenching of carbonyl metabolites in triplet state by alkylated sorbates, esterified or peptide derivatives
• Grantee: Thiago da Mata Viola Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/24515-6 - Sources, targets and biological responses of triplet species and singlet oxygen: pathogenicity and quenching
• Grantee: Luiz Duarte Ramos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral