Screening of yeast co-culture using crude hydrolysate for co-fermentation of pentose and hexose

Author(s)

Sandri, Juliana P. ; Milessi, Thais S. ; Zangirolami, Teresa C. ; Mussatto, Solange I.

• Document type: Journal article
• Source: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. N/A, p. 15-pg., 2023-08-08.

Abstract

The use of all sugars from biomass fractionation - that is, hexoses and pentoses - is essential to develop feasible processes in a biorefinery context. Hexoses are converted into different products by most microorganisms. The main issue is the conversion of pentoses, especially when mixed with hexoses. Improving sugar conversion in co-fermentation is a major challenge, which was explored in this work. Co-culture using Saccharomyces cerevisiae and evolved Kluyveromyces marxianus strains was evaluated by screening using 24 deep-well plates. Glucose depletion and 83% of xylose conversion were achieved with supplemented hemicellulosic hydrolysate medium (medium volume corresponding to 2/5 of deep-well volume), and initial cell concentrations of 2.5 g L-1 for each yeast, incubated at 150 rpm. The screening strategy provided reliable data that confirmed the co-culture as a promising approach for the co-assimilation of biomass-derived sugars. The importance of aeration control for effective xylose assimilation was also pointed out. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants