| Full text | |
| Author(s): |
Pierry, Paulo Marques
;
Uceda-Campos, Guillermo
;
Feitosa-Junior, Oseias Rodrigues
;
Martins-Junior, Joaquim
;
de Santana, Wesley Oliveira
;
Della Coletta-Filho, Helvecio
;
Zaini, Paulo Adriano
;
da-Silva, Aline Maria
Total Authors: 8
|
| Document type: | Journal article |
| Source: | ROPICAL PLANT PATHOLOG; v. 45, n. 3, p. 19-pg., 2020-06-10. |
| Abstract | |
Plasmids are a major source of horizontal gene transfer among bacteria contributing to their evolution and ecology. The known collection of plasmids carried by the plant pathogenXylella fastidiosaincreases as new strains from different origins and hosts are sampled and sequenced. Here we performed an extensive comparison of 61 publicly available sequences annotated as plasmids belonging to 38X. fastidiosastrains isolated from different plant species and distinct geographical locations. Twenty-six strains exhibited at least one plasmid and up to four plasmids were found in a single strain. Plasmids sizes varied widely from 1.3 kbp to 64.3 kbp, ranging from 1 to 70 protein-coding sequences (CDS) encompassing 324 orthologs. Based on the presence of specific mobility proteins such as relaxases and type 4 secretion system-related genes, respectively 40 and 8 of theX. fastidiosaplasmids were classified as conjugative and mobilizable, while 13 were classified as non-mobilizable.X. fastidiosaplasmids did not carry known antibiotic resistance or virulence genes, and their stability seems to take advantage of toxin/antitoxin systems. The comparative analyses described here revealed similarity among plasmids ofX. fastidiosafrom different subspecies, geographical regions, and hosts, as well as with sequences found in plasmids from other bacterial species. Altogether, our results provide anin silicoanalysis ofX. fastidiosaplasmid content and their main features, with applications in future studies of epidemiology, ecology, and evolution of this phytopathogen. (AU) | |
| FAPESP's process: | 11/01217-8 - Characterization of Citrus sinensis response to Xylella fastidiosa infection and phylogenomics of its different strains. |
| Grantee: | Joaquim Martins Junior |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/13527-1 - Genome sequencing e comparative analysis of a Xylella fastidiosa strain. |
| Grantee: | Paulo Marques Pierry |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 08/11703-4 - Identification of genes potentially involved in adaptation, evolution and virulence of Xylella fastidiosa |
| Grantee: | Aline Maria da Silva |
| Support Opportunities: | Regular Research Grants |