Zn-Al layered double hydroxides induce embryo malformations and impair locomotion behavior in Danio rerio

Author(s)

Carneiro, Diana ; Damasceno, Evila Pinheiro ; Ferreira, Violeta ; Charlie-Silva, Ives ; Tedim, Joao ; Maia, Frederico ; Loureiro, Susana ; Martins, Roberto ; Pavlaki, Maria D.

• Document type: Journal article
• Source: NANOIMPACT; v. 30, p. 12-pg., 2023-02-27.

Abstract

Layered double hydroxides (LDHs) are stimuli-responsive anionic nanoclays. The vast possibilities of using LDHs can lead to their existence in the ecosystem, raising a question of potential ecological concern. However, little is known about the effect of these nanomaterials on freshwater organisms. The present study aimed to assess the ecotoxicological effects of Zinc-Aluminium LDH-nitrate (Zn-Al LDH-NO3) in zebrafish (Danio rerio) early life stages. The endpoints measured were mortality, malformations and hatching rate after exposure of D. rerio embryos and larvae to Zn-Al LDH-NO3 following the OECD 236 guideline. The behavioral, biochemical (markers of oxidative stress and neurotoxicity), and molecular (at DNA level) alterations were also assessed using sub-lethal concentrations. No observable acute effects were detected up to 415.2 mg LDH/L while the 96 h-LC50 was estimated as 559.9 mg/L. Tested LDH caused malformations in D. rerio embryos, such as pericardial edema, incomplete yolk sac absorption and tail deformities (96 h-EC50 = 172.4 mg/L). During the dark periods, the locomotor behavior in zebrafish larvae was affected upon Zn-Al LDH-NO3 exposure. However, no significant biochemical and molecular changes were recorded. The present findings suggest that Zn-Al LDH-NO3 can be regarded as a non-toxic nanomaterial towards D. rerio (E/LC50 > > 100 mg/L) although impairment of the locomotion behavior on zebrafish embryos can be expected at concentrations below 100 mg/L. (AU)

• FAPESP's process: 19/19939-1 - Effects of marine pharmaceuticals in ZFL cell line
• Grantee: Ives Charlie da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/07098-0 - Effects of marine pharmaceuticals in zebrafish and ZFL cell line - emphasis
• Grantee: Leticia Veras Costa Lotufo
• Support Opportunities: Regular Research Grants