Improving Saccharomyces cerevisiae acid and oxidative stress resistance using a prokaryotic gene identified by functional metagenomics

Author(s)

Alves, Luana de Fatima ; Bortolucci, Jonata ; Reginatto, Valeria ; Guazzaroni, Maria-Eugenia ; Mussatto, Solange I.

• Document type: Journal article
• Source: HELIYON; v. 9, n. 4, p. 12-pg., 2023-04-01.

Abstract

Innovations in obtaining products from lignocellulosic biomass have been largely based on the improvement of microorganisms and enzymes capable of degrading these materials. To complete the whole process, microorganisms must be able to ferment the resulting sugars and tolerate high concentrations of product, osmotic pressure, ion toxicity, temperature, toxic compounds from lignocellulose pretreatment, low pH, and oxidative stress. In this work, we engineered laboratory and industrial Saccharomyces cerevisiae strains by combining a gene (hu) recovered from a metagenomic approach with different native and synthetic promoters to obtain improved acid and oxidative stress resistance. Laboratorial strains harboring hu gene under the control of the synthetic stress responsive PCCW14v5 showed increased survival rates after 2 h exposure to pH 1.5. The hu gene was also able to significantly enhance the tolerance of the industrial strain to high concentrations of H2O2 when combined with PTEF1, PYGP1 or PYGP1v7 after 3 h exposure. (AU)

• FAPESP's process: 21/01748-5 - Discovery and development of new non-model bacterial chassis for biotechnological applications
• Grantee: María Eugenia Guazzaroni
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/06323-4 - New tools for improving metagenomic cellulase screenings
• Grantee: Luana de Fátima Alves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/03168-3 - Expression of enzymes related to 1,3-propanediol sustainable production by Clostridium strains from glycerol and other substrates
• Grantee: Jonatã Bortolucci
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/16191-3 - Engineering Saccharomyces cerevisiae strains to improve acid- and lignocellulosic inhibitor-tolerance
• Grantee: Luana de Fátima Alves
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate