Expression of enzymes related to 1,3-propanediol sustainable production by Clostridium strains from glycerol and other substrates

• Grant number: 20/03168-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: September 01, 2020
• End date until: December 31, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Valeria Reginatto Spiller
• Grantee: Jonatã Bortolucci
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 21/06757-2 - Transformation of Clostridium beijerinckii Br21 for insertion of the gene dhaB, to encode the enzyme glycerol dehydratase, BE.EP.MS

Abstract

Increasing interest in economic practices based on the use of renewable and residual materials have made biotechnological processes some of the most attractive alternatives to add value to such raw materials. For example, biodiesel production from the recycling of oils and fats generates glycerol as a by-product, which in turn has been studied as a carbon source in several bioprocesses, including glycerol conversion to 1,3-propanediol (1,3-PDO) by bacteria of the genus Clostridium. Glycerol is metabolized by two routes: the oxidative route involves the enzymes glycerol dehydrogenase and dihydroxyacetone kinase, whereas the reductive route relies on glycerol dehydratase and 1,3-propanediol oxidoreductase. The final yield of the metabolite 1,3-PDO depends on the gene expression of the enzymes. The use of xylose as a co-substrate increases the formation of reducing equivalents and hence the production of 1,3-PDO. However, this compound alters the enzyme expression profile. In addition to glycerol and carbohydrates, some clostridia can capture CO2, providing an even greater yield of the desired product. Thus, this investigation aims to expand knowledge about the glycerol metabolism pathways by investigating gene expression by qRT-PCR, with a view to the subsequent transformation of the microorganism. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, LUANA DE FATIMA; BORTOLUCCI, JONATA; REGINATTO, VALERIA; GUAZZARONI, MARIA-EUGENIA; MUSSATTO, SOLANGE I.. Improving Saccharomyces cerevisiae acid and oxidative stress resistance using a prokaryotic gene identified by functional metagenomics. HELIYON, v. 9, n. 4, p. 12-pg., 2023-04-01. (21/01748-5, 16/06323-4, 20/03168-3, 18/16191-3)

BORTOLUCCI, JONATA; ZANI, ANA CLARA BONIZOL; DE GOUVEA, PAULA FAGUNDES; DINAMARCO, TAISA MAGNANI; REGINATTO, VALERIA. A non-solventogenic Clostridium beijerinckii strain lacking acetoacetate decarboxylase assimilates acetate and accumulates butyrate. BIOMASS & BIOENERGY, v. 172, p. 8-pg., 2023-03-31. (16/05578-9, 18/12471-1, 22/04024-0, 18/10296-8, 20/03168-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORTOLUCCI, Jonatã. Production of 1,3-propanediol by Clostridium beijerinckii Br21 from glycerol: gene expression of the main enzymes involved and genetic manipulation by transformation. 2023. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.