Comparison of hydrophilic and hydrophobic nano topographic surfaces of titanium alloys on pre-osteoblastic cell interaction

Author(s)

de Oliveira, Diego Pedreira ; Micocci, Kelli Cristina ; Almeida, Gustavo Foresto Brito de ; Otuka, Adriano Jose Galvani ; Mendonca, Cleber Renato ; Selistre-de-Araujo, Heloisa Sobreiro ; Bolfarini, Claudemiro

• Document type: Journal article
• Source: BIOMEDICAL PHYSICS & ENGINEERING EXPRESS; v. 9, n. 4, p. 12-pg., 2023-07-01.

Abstract

This work aimed to assess the influence of different structured substrates with hydrophilic and hydrophobic properties on micro and nano topographies developed on titanium alloys over pre-osteoblastic cell behavior. Nano topography influences small dimension levels of cell morphology by inducing filopodia formation in cell membranes, irrespectively to the wettability behavior of the surface. Therefore, micro and nanostructured surfaces of titanium-based samples using different techniques of surface modification processing, such as chemical treatments, micro-arc anodic oxidation (MAO), and MAO combined to laser irradiation were developed. Isotropic and anisotropic texture morphologies, wettability, topological parameters and compositional alterations were measured after the surface treatments. Finally, cell viability, adhesion and morphological responses were assessed to investigate the influence of distinct topologies on osteoblastic cells aiming to encounter the conditions to better promote mineralization events. Our study demonstrated that the hydrophilic behavior improves cell adhesion, amplified when effective surface area increases. Surfaces presenting nano topography have a direct influence on cell morphology and play a key role for filopodia formation. (AU)

• FAPESP's process: 11/12399-0 - Femtosecond pulses applied to nonlinear optics: spectroscopy, pulse shaping and microfabrication
• Grantee: Cleber Renato Mendonça
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/06258-0 - Development of surfaces anodically oxidized of titanium alloys and modified femtosecond laser and fatigue resistant
• Grantee: Diego Pedreira de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral