| Full text | |
| Author(s): |
Berto, Gabriela L.
;
Mattos, Bruno D.
;
Velasco, Josman
;
Zhao, Bin
;
Segato, Fernando
;
Rojas, Orlando J.
;
Arantes, Valdeir
Total Authors: 7
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 243, p. 9-pg., 2023-06-13. |
| Abstract | |
Enzymatic processing is considered a promising approach for advancing environmentally friendly industrial processes, such as the use of endoglucanase (EG) enzyme in the production of nanocellulose. However, there is ongoing debate regarding the specific properties that make EG pretreatment effective in isolating fibrillated cellulose. To address this issue, we investigated EGs from four glycosyl hydrolase (GH) families (5, 6, 7, and 12) and examined the roles of the three-dimensional structure and catalytic features, with a focus on the presence of a carbohydrate binding module (CBM). Using eucalyptus Kraft wood fibers, we produced cellulose nanofibrils (CNFs) through mild enzymatic pretreatment, followed by disc ultra-refining. Comparing the results with the control (without pretreatment), we observed that GH5 and GH12 enzymes (without CBM) reduced fibrillation energy by approximately 15 %. The most significant energy reduction, 25 and 32 %, was achieved with GH5 and GH6 linked to CBM, respectively. Notably, these CBM-linked EGs improved the rheological properties of CNF suspensions without releasing soluble products. In contrast, GH7-CBM exhibited significant hydrolytic activity, resulting in the release of soluble products, but did not contribute to a reduction in fibrillation energy. This discrepancy can be attributed to the large molecular weight and wide cleft of GH7-CBM, which led to the release of soluble sugars but had little impact on fibrillation. Our findings suggest that the improved fibrillation observed with EG pretreatment is primarily driven by efficient enzyme adsorption on the substrate and modification of the surface viscoelasticity (amorphogenesis), rather than hydrolytic activity or release of products. (AU) | |
| FAPESP's process: | 15/02862-5 - NANOCEL - development of enzyme mixtures for the preparation of nanocelluloses by a biohibrid process |
| Grantee: | Valdeir Arantes |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse |
| Grantee: | Fernando Segato |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 21/07023-2 - Development of an enzyme-based platform technology for production and modulation of nanocellulose: validation for biomedical and food packaging applications |
| Grantee: | Valdeir Arantes |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 21/06679-1 - Enzymatic oxidation of sugarcane bagasse 2: exploring the interaction between LPMOs and its redox partner CDHs aiming for the development of more efficient enzymes for insertion into an engineered cell factory |
| Grantee: | Fernando Segato |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants - Phase 2 |