Sugarcane bagasse pretreated by different technologies used as support and carbon source in solid-state fermentation by Aureobasidium pullulans LB83 to produce bioemulsifier

Author(s)

da Costa, Rogger Alessandro Mata ; Rubio-Ribeaux, Daylin ; Carneiro, Bruna Curry ; Franco, Paulo Marcelino ; Mendes, Geissy de Azevedo ; da Silva, Isis Lee ; dos Anjos, Virgilio de Carvalho ; dos Santos, Julio Cesar ; Tiso, Till ; da Silva, Silvio Silverio

• Document type: Journal article
• Source: BIOMASS CONVERSION AND BIOREFINERY; v. N/A, p. 14-pg., 2023-02-10.

Abstract

Bioemulsifier production by Aureobasidium pullulans LB83 from pretreated sugarcane bagasse was evaluated in solid-state fermentation. Alkaline (0.2 mol.L-1 NaOH; 12 min) and hydrodynamic cavitation (0.5 mol.L-1 NaOH, 25 min) pretreatments showed a maximum lignin removal of 55.1 % and 44.7 %, respectively. Pretreatment biomass effectivity was assessed by X-ray diffraction, FT-MIR, FTIR-NIR, and RAMAN techniques. Maximum kerosene emulsification indexes of 65.8 % and 41.3 % were obtained in solid-state fermentation, respectively, for alkaline and hydrodynamic cavitation pretreated sugarcane bagasse. Synthesis of cellulases was observed in fermentation, showing maximum values of endoglucanase and exoglucanase, respectively, of 2.25 U.g(-1) and 1.39 U.g(-1), for alkaline pretreated sugarcane bagasse, and 2.43 U.g(-1) and 1.45 U.g(-1), for hydrodynamic cavitation-pretreated sugarcane bagasse. The biomolecule was characterized as a mixture of mannitol and arabitol-type liamocin. Microorganism was able to produce bioemulsifier using sugarcane bagasse as support and carbon source in solid-state fermentation, thus showing the potential of this system to obtain value-added products from this biomass. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 20/06323-0 - Biosurfactants production by solid state fermentation using Aureobasidium pullulans with sugarcane bagasse as a substrate: from biomass pretreatment to bioreactor design
• Grantee: Daylin Rubio Ribeaux
• Support Opportunities: Scholarships in Brazil - Post-Doctoral