Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

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Miranda, Luis Fernando B. ; Lima, Carolina V. ; Pagin, Rafaela ; Costa, Raphael C. ; Pereira, Marta Maria A. ; de Avila, Erica D. ; Bertolini, Martinna ; Retamal-Valdes, Belen ; Shibli, Jamil A. ; Feres, Magda ; Barao, Valentim A. R. ; Souza, Joao Gabriel S.

• Document type: Journal article
• Source: JOURNAL OF PROTEOME RESEARCH; v. 22, n. 3, p. 14-pg., 2023-03-03.

Abstract

The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 mu m filtered, 0.45 mu m filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (approximate to 19%) were exclusive. Saliva processing reduced identified proteins to 222 (approximate to 80%) for the 0.22 mu m group, 219 (approximate to 79%) for the 0.45 mu m group, and 201 (approximate to 72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability. (AU)

• FAPESP's process: 21/09434-0 - A novel antimicrobial drug-delivery coating for percutaneous implant devices
• Grantee: Érica Dorigatti de Avila
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 18/20719-3 - A novel antimicrobial drug-delivery coating for percutaneous implant devices
• Grantee: Érica Dorigatti de Avila
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 20/10436-4 - Synthesis, characterization and biological performance of pH-sensitive nanopolymers produced by molecular printing for the treatment of peri-implant infections
• Grantee: Raphael Cavalcante Costa
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/10762-1 - Vascular regenerative MAA-based material film on antimicrobial [PAA/PLL]10/TC/anionic B-CD coating for percutaneous implants devices
• Grantee: Marta Maria Alves Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)