Use of Reflectometer Optipen to assess the preventive effect of a sugarcane cystatin on initial dental erosion in vivo

Author(s):Show less -

Pela, Vinicius Taioqui ; Ventura, Talita Mendes Oliveira ; Taira, Even Akemi ; Thomassian, Larissa Tercilia Grizzo ; Brito, Leonardo ; Matuhara, Yan Eiji ; Henrique-Silva, Flavio ; Groisman, Sonia ; Carvalho, Thiago Saads ; Lussi, Adrian ; Buzalaf, Marilia Afonso Rabelo

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 141, p. 6-pg., 2023-03-17.

Abstract

The sugarcane cystatin 5 (CaneCPI-5) showed protection against erosion and erosive tooth wear (ETW) under several protocols. However, evaluating these conditions in vivo is hard due to the lack of a suitable device. The aim of this study was to use clinically the relative surface reflection intensity (%SRI) by the Reflectometer Optipen to assess the acquired pellicle engineering with CaneCPI-5 rinse for the prevention of initial erosion in vivo. Nine volunteers were distributed in three cross-over phases, according to the rinse used, as follows: 1) Deionized water (negative control); 2) Elmex (R) (800 ppm Sn2+, 500 ppm F-; positive control); 3) 0.1 mg/mL CaneCPI-5. The following experimental steps were performed: Initially, the volunteers received prophylaxis and the initial %SRI was performed. Subsequently, they rinsed with the solutions (10 mL; 1min), followed by the formation of the acquired enamel pellicle (AEP; 120min). After, the erosive challenge with citric acid 1%, pH 2.5 (10 mu L; 10s) was performed (in isolation) on the buccal surface of the maxillary central incisors (right and left). The calcium present in the acid was analyzed by Arsenazo III method. Finally, the final %SRI was performed. Data were analyzed by Kruskal-Wallis/Dunn's tests and Spearman's correlation were used (p < 0.05). For both variables, the negative control led to significantly less protection (lower reflectivity and higher calcium release) in comparison with the other groups. The best protection (higher reflectivity and lower calcium release) was observed for the Elmex (R) and the CaneCPI-5 groups, with no significant differences between them (p < 0.05). There was a significant correlation between both analyzes. The Reflectometer Optipen demonstrated to be a good device to assess clinically. Moreover, CaneCPI-5 rinse proved effective through acquired pellicle engineering against initial erosion in vivo. (AU)

• FAPESP's process: 17/04857-4 - Acquired pellicle engineering for control of dental erosion: in situ evaluation of protective potential by a new cistatin derived from sugar cane
• Grantee: Vinícius Taioqui Pelá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/08437-5 - Acquired pellicle engineering to protect against dental erosion: use of a new hand-held optical reflectometer to evaluate the protective role of proteins/peptides
• Grantee: Vinícius Taioqui Pelá
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants