Determination of Co2+ ions in blood samples: A multi-way sensing based on NH2-rich carbon quantum dots

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Oliveira, J. J. P. ; Carneiro, S. V. ; Cruz, A. A. C. ; Fechine, L. M. U. D. ; Michea, S. ; Antunes, R. A. ; Neto, M. L. A. ; Moura, T. A. ; Cesar, C. L. ; Carvalho, H. F. ; Paschoal, A. R. ; Lopes, G. S. ; Freire, R. M. ; Fechine, P. B. A.

• Document type: Journal article
• Source: DYES AND PIGMENTS; v. 215, p. 12-pg., 2023-03-22.

Abstract

Multiple forms of detecting Co2+ are reported in this work to quantify these ions in real blood plasma samples. Carbon Quantum Dots (CQDs) were used as a fluorescent nanoprobe. The CQDs were obtained from a bottom-up approach using a hydrothermal method and choline chloride and branched poly(ethyleneimine) as precursor molecules. Several spectroscopic and structural characterizations were performed. The efficient fluorescence quenching of CQDs related to the Co2+ ion was used for the detection of the analyte, generating a sensing strategy with a limit of detection (LOD) of 0.98 mu mol L-1. Furthermore, the interaction between the Co2+ ion with the CQDs resulted in the color change of the solution from colorless to pale yellow. Thus, a colorimetric Co2+ sensor was also developed, since there was an absorption band at 315 nm attributed to the formation of the complex CQD + Co2+. The colorimetric method showed an excellent sensitivity to Co2+, with a LOD of 3.01 mu mol L-1. In addition, Principal Component Analysis (PCA) together with Linear Discriminant Analysis (LDA) were used successfully to distinguish different concentrations of Co2+ and different interfering ions present in the solution. Finally, a real sample of blood plasma was properly treated and doped with different concentrations of Co2+, which was successfully quantified via fluorescent method. Therefore, the CQDs obtained in this work are a powerful and versatile Co2+ detection tool. (AU)

• FAPESP's process: 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology
• Grantee: Hernandes Faustino de Carvalho
• Support Opportunities: Research Projects - Thematic Grants