UV-C light promotes the reductive cleavage of disulfide bonds in beta-Lactoglobulin and improves in vitro gastric digestion

Author(s)

da Silva, Juliana F. ; Morais, Aline T. do B. ; Santos, Willy G. ; Ahrne, Lilia M. ; Cardoso, Daniel R.

• Document type: Journal article
• Source: Food Research International; v. 168, p. 11-pg., 2023-03-28.

Abstract

beta-Lactoglobulin (beta-Lg) is the main protein in whey and is known for its allergenicity and resistance to the digestion of pepsin and trypsin. The UV-C photoinduced cleavage of disulfide bonds in beta-Lactoglobulin, as promoted by excitation of tryptophan residues (Trp), is shown to induce changes in the protein's secondary structure, significantly reducing the protein's resistance to pepsin digestion. The UV-C light-induced changes in the protein secondary structure are marked by an increase in the contribution of beta-sheet and alpha-helix structures with a concomitantly smaller contribution of the beta-turn structural motif. The photoinduced cleavage of disulfide bonds in beta-Lg has an apparent quantum yield of Phi = 0.0015 +/- 0.0003 and was shown by transient absorption laser flash photolysis to arise by two different pathways: a) the reduction of the disulfide bond Cys(66)Cys(160) occurs by direct electron transfer from the triplet-excited 3Trp to the disulfide bond due to the existence of a CysCys/Trp triad (Cys(66)Cys(160)/Trp(61)) and b) the reduction of the buried Cys(106)Cys(119) disulfide bond involves a reaction with a solvated electron originated by the photoejection of electrons from the triplet-excited (3)Trp decay. The in vitro gastric digestion index for UV-C-treated beta Lg is revealed to have increased significantly by 36 +/- 4 % and 9 +/- 2 % under simulated elderly and young adult digestive conditions, respectively. When compared to the native pro-tein, the peptide mass fingerprint profile of digested UV-C-treated beta-Lg shows a higher content and variety of peptides, including the production of some exclusive bioactive peptides such as PMHIRL and EKFDKALKALPMH. (AU)

• FAPESP's process: 17/01189-0 - Novel aging: technologies and solutions to manufacture novel dairy products for healthy aging
• Grantee: Daniel Rodrigues Cardoso
• Support Opportunities: Research Projects - Thematic Grants