Relating biomass composition and the distribution of metabolic functions in the co-fermentation of sugarcane vinasse and glycerol

Author(s)

de Menezes, Camila Aparecida ; de Souza Almeida, Priscilla ; Delforno, Tiago Palladino ; de Oliveira, Valeria Maia ; Sakamoto, Isabel Kimiko ; Varesche, Maria Bernadete Amancio ; Silva, Edson Luiz

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 48, n. 24, p. 17-pg., 2023-03-19.

Abstract

This study aimed to determine the effect of increasing the organic loading rate (OLR) from 60 to 90 and 120 kg COD m-3 d-1 in the co-fermentation of glycerol and sugarcane vinasse (50%: 50% proportion on a COD basis) in a thermophilic anaerobic fluidized bed reactor (55 degrees C) at the fixed hydraulic retention time of 4 h. The highest values of hydrogen production rate (1851 mL H2 d-1 L-1bed) and yield (0.29 mmol H2 g-1 CODadded) were found at 120 kg COD m-3 d-1 and coincided with butanoate as a major liquid metabolite (2620 mg L-1). The reverse b-oxidation of lactate into butanoate contributed to its synthesis and was linked to synergism between Clostridium (relative abundance of 77.8%) and Lacti-caseibacillus (7.2%) in the reactor. The identification of the butyryl-CoA/acetate-CoA transferase gene, which may have catalyzed the conversion of butyryl-CoA into buta-noate using acetate as an acceptor, also supported this. (c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants