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Identification of target genes regulated by the SHINE transcription factor in monocot plants

Grant number: 14/08468-4
Support type:Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
Duration: November 01, 2014 - October 31, 2017
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Michael dos Santos Brito
Grantee:Michael dos Santos Brito
Home Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
Assoc. researchers:Paula Macedo Nobile ; Paulo Mazzafera ; Silvana Aparecida Creste Dias de Souza
Associated scholarship(s):16/23437-3 - Characterizing the transcription factor SHN regulation targets by ChIP-qPCR , BP.IC
14/23017-9 - Identification of target genes regulated by the SHINE transcription factor in monocot plants, BP.JP.BIOEN

Abstract

Plants with high content of lignocellulosic biomass are being selected for the production of second-generation ethanol. One of the main bottleneck to use biomass for bioethanol production is lignin, a polymeric component of the secondary cell wall which confers recalcitrance and inhibits fermenting microorganisms growth. In this scenario, transgenic plants with less or altered lignin composition are an attractive alternative to solve the challenges imposed by lignin. Among many genes potentially suitable for lignin metabolic engineering, transcription factors (TFs) controlling genes of the lignin biosynthesis are primary targets. On the other hand, the most knowledge regarding TFs and lignin biosynthesis were obtained using Arabidopsis as a model plant. As published previously sometimes a TF can regulate determined pathway in a specie-specific manner. Recently one example of this regulation was published with SHINE (SHN) transcription factor. SHINE represents a TF member of AP2 family, has been characterized in Arabidopsis and rice. SHN has shown different functions in these two plants, being related with cutin wax synthesis in Arabidopsis and as a master regulator of secondary cell wall biosynthesis in rice. It was shown, that rice plants overexpressing AtSHN2, presented the inhibition of the expression of lignin-related genes and induction of genes for cellulose biosynthesis. So far, nothing is known about this class of TFs and their role in regulating the biosynthesis of lignin in other monocots like sugarcane, maize or sorghum. In a RNAseq approach, using two contrasting sugarcane genotypes for lignin content, we found the homologue of SHN in sugarcane. Previously, it was initiated a Chromatin immunoprecipitation assay (ChIP) in sugarcane aiming to validate SHN targets in sugarcane. For this it was developed an antibody antiSHN that was also designed to recognize SHN proteins in other monocots like maize and sorghum. The objective here is to unveil what are the targets of SHN in monocot plants (sugarcane, maize and sorghum). The results we aim to obtain will be compared with those previously obtained in rice, to unravel the role of this TF in secondary cell wall construction in monocots. In a longer perspective, this TF may be useful to manipulate lignin and cellulose biosynthesis in monocot plants used for the production of second generation bioethanol. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOER MARTINS, ALEXANDRE PALMA; BRITO, MICHAEL DOS SANTOS; MAYER, JULIANA LISCHKA S.; PORTILLA LLERENA, JUAN PABLO; OLIVEIRA, JASMIM FELIPE; TAKAHASHI, NATALIA CONGALVES; CARLIN, SAMIRA DOMINGUES; ALINE FLORES BORGES, DENISELE NEUZA; ANDRADE, LARISSA MARA; PEIXOTO-JUNIOR, RAFAEL FAVERO; DE SOUZA GOLDMAN, MARIA HELENA; MAZZAFERA, PAULO; CRESTE, SILVANA; NOBILE, PAULA MACEDO. Ectopic expression of sugarcane SHINE changes cell wall and improves biomass in rice. BIOMASS & BIOENERGY, v. 119, p. 322-334, DEC 2018. Web of Science Citations: 1.
CESARINO, IGOR; SIMOES, MARCELLA SIQUEIRA; BRITO, MICHAEL DOS SANTOS; FANELLI, AMANDA; SILVA, TATIANE DA FRANCA; ROMANEL, ELISSON. Building the wall: recent advances in understanding lignin metabolism in grasses. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 11 NOV 2016. Web of Science Citations: 10.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180704168 - Instituto Agronômico de Campinas (IAC) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - October 2018, 03