Ectopic expression of sugarcane SHINE changes cell wall and improves biomass in rice

Author(s):Show less -

Boer Martins, Alexandre Palma ^{[1, 2]} ; Brito, Michael dos Santos ^{[2, 3]} ; Mayer, Juliana Lischka S. ^[4] ; Portilla Llerena, Juan Pablo ^[4] ; Oliveira, Jasmim Felipe ^[4] ; Takahashi, Natalia Congalves ^{[2, 5]} ; Carlin, Samira Domingues ^[2] ; Aline Flores Borges, Denisele Neuza ^[4] ; Andrade, Larissa Mara ^{[1, 2]} ; Peixoto-Junior, Rafael Favero ^{[1, 2]} ; de Souza Goldman, Maria Helena ^{[1, 6]} ; Mazzafera, Paulo ^{[4, 7]} ; Creste, Silvana ^{[1, 2]} ; Nobile, Paula Macedo ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[2] Inst Agron IAC, Ctr Cana, Rodovia Antonio Duarte Nogueira, Km 321, BR-14032800 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim, 330 Sala 201, BR-12231280 Sao Jose Dos Campos, SP - Brazil

^[4] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Campinas, SP - Brazil

^[5] UNESP, Dept Melhoramento Genet, Jaboticabal, SP - Brazil

^[6] Univ Sao Paulo, Fac Filosofia, Dept Biol Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[7] Univ Sao Paulo, Dept Prod Vegetal, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: BIOMASS & BIOENERGY; v. 119, p. 322-334, DEC 2018.
• Web of Science Citations: 1

Abstract

One of the major challenges involved in biofuel production from plant biomass is increasing the saccharification efficiency impaired by cell wall recalcitrance. The sugarcane transcription factor ShSHN1 was overexpressed in rice to evaluate its impacts on cell wall composition and potential for the development of refined lignocellulosic feedstocks for biofuel manufacturing. Plant phenotyping included biomass and cell wall component determination. Additionally, gene expression of biosynthetic cell wall polymers and wax/cutin-related genes were evaluated. ShSHN1 ectopic overexpression promoted an increase in biomass (91-340%), pectin (26-209%), cellulose content (10-22%) and saccharification efficiency (5-53%) in rice transgenic plants, as well as a reduction in the lignin content (17-35%) and an altered lignin composition. Such an increase in pectin is a new finding and opens up new research pathways for cell wall manipulation of energy crops. Furthermore, ShSHN1 overexpression led to changes in the expression patterns of all genes analyzed, suggesting a possible relation between ShSHN1 and the regulatory mechanisms involved in secondary plant cell wall formation. Saccharification improvement and biomass increase made the perfect combination for second generation ethanol production. This synergistic gain is discussed based on structural polysaccharides and lignin modification. Phenotypes and gene expression analysis provide insights into possible SHINE mechanisms of action and biological role in cell wall component biosynthesis. These results demonstrate the great potential of ShSHN1 to improve grass feedstocks as a source of energy. (AU)

• FAPESP's process: 14/23017-9 - Identification of target genes regulated by the SHINE transcription factor in monocot plants
• Grantee: Michael dos Santos Brito
• Support Opportunities: Scholarships in Brazil - BIOEN - Young Researchers
• FAPESP's process: 14/08468-4 - Identification of target genes regulated by the SHINE transcription factor in monocot plants
• Grantee: Michael dos Santos Brito
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 16/14309-1 - Qualitative and quantitative analysis of the anatomical structure of genetically modified plants for the lignin content
• Grantee: Jasmim Felipe de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/19214-0 - Sugarcane gene functional analysis in transgenic rice and lignin modified sugarcane in order to cellulosic ethanol production
• Grantee: Paula Macedo Nobile
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/09422-8 - Sugarcane gene functional analysis in transgenic rice and lignin modified sugarcane in order to cellulosic ethanol production
• Grantee: Paula Macedo Nobile
• Support Opportunities: Scholarships in Brazil - Young Researchers