| Full text | |
| Author(s): |
Chiarelli-Neto, Orlando
;
Garcez, Michelle Lima
;
Pavani, Christiane
;
Martins, Waleska
;
Castro, Fernanda Cristina de Abreu Quintela
;
Ambrosio, Roberta Passamani
;
Meotti, Flavia Carla
;
Baptista, Mauricio S.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 239, p. 9-pg., 2023-01-10. |
| Abstract | |
UV-A radiation affects skin homeostasis by promoting oxidative distress. Endogenous photosensitizers in the dermis and epidermis of human skin absorb UV-A radiation forming excited states (singlet and triplet) and reactive oxygen species (ROS) producing oxidized compounds that trigger biological responses. The activation of NF-kB induces the expression of pro-inflammatory cytokines and can intensify the generation of ROS. However, there is no studies evaluating the cross talks between inflammatory stimulus and UV-A exposure on the levels of redox misbalance and inflammation. In here, we evaluated the effects of UV-A exposure on J774 macrophage cells previously challenged with LPS in terms of oxidative distress, release of pro-inflammatory cytokines, and activation of regulated cell death pathways. Our results showed that LPS potentiates the dose-dependent UV-A -induced oxidative distress and cytokine release, in addition to amplifying the regulated (autophagy and apoptosis) and non-regulated (necrosis) mechanisms of cell death, indicating that a previous inflammatory stimulus potentiates UV-A-induced cell damage. We discuss these results in terms of the current-available skin care strategies. (AU) | |
| FAPESP's process: | 18/22922-0 - Modulation of autophagy by biochemical and photochemical stress: therapeutic implications |
| Grantee: | Waleska Kerllen Martins Gardesani |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 18/23257-0 - Modulation of biochemical and photochemical parallel damages: implications on pro-survival autophagy |
| Grantee: | Maryana do Nascimento da Silva |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |