New insights into the protective effect of statherin-derived peptide for different acquired enamel pellicle formation times on the native human enamel surfaces

Author(s)

Ventura, Talita Mendes Oliveira ; Buzalaf, Marilia Afonso Rabelo ; Baumann, Tommy ; Pela, Vinicius Taioqui ; Niemeyer, Samira Helena ; Crusca, Edson ; Marchetto, Reinaldo ; Lussi, Adrian ; Carvalho, Thiago Saads

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 148, p. 7-pg., 2023-04-01.

Abstract

Objectives: This study evaluated the protective impact of acquired enamel pellicle (AEP) engineering with statherin-derived peptide (StatpSpS), considering different AEP formation times.Design: A total of 120 native human enamel specimens were divided into 2 main groups: 1) No AEP engineering and 2) AEP engineering with StatpSpS (pretreatment for 1 min; 37 degrees C, under agitation). Each group was further divided into 4 subgroups: No pellicle, or 1, 60-and-120 min AEP formation times (human saliva; 37 degrees C). The specimens were then subjected to an erosive challenge (1% citric acid; pH 3.6; 1 min; 25 degrees C). This procedure was repeated for 5 cycles. Relative surface reflection intensity (%SRI) was measured and scanning electron micro-scopy (SEM) of the enamel surface was done.Results: All AEP engineering groups protected against initial dental erosion in comparison with No pellicle (p < 0.001), likewise all groups with AEP, independent of engineering or formation times (p 0.001). Furthermore, engineering with StatpSpS even without the presence of AEP protected the enamel when compared to the No engineering/No pellicle group (p < 0.0001). No difference was observed regarding the protection from the different AEP formation times (p > 0.05). Regarding the SEM analysis, in the "No AEP engineering & No AEP" group, a more severe effect of citric acid was observed, with more enamel prism heads and scratches on the surface when compared with the other groups.Conclusions: AEP provides almost instant protection at formation times even as short as 1 min, protecting the native enamel against erosion. Treatment with StatpSpS by itself provides similar protection as the AEP. (AU)

• FAPESP's process: 19/16815-0 - Protective role of acquired enamel pellicle formed for different times and of Statherin peptide against initial dental erosion: evaluation using a new hand-held optical reflectometer
• Grantee: Talita Mendes Oliveira Ventura
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants