Biosynthesis of silver nanoparticles of Tribulus terrestris food supplement and evaluated antioxidant activity and collagenase, elastase and tyrosinase enzyme inhibition: In vitro and in silico approaches

Author(s)

Gomes, Amanda da Costa ; Zibordi, Laura Camargo ; Granero, Filipe Oliveira ; Ximenes, Valdecir Farias ; Pavan, Nathalia Mariana ; Silva, Luciana Pereira ; Sonvesso, Cibely da Silva Martin ; Job, Aldo Eloizo ; Nicolau, Nilson ; Silva, Regildo Marcio Goncalves da

• Document type: Journal article
• Source: FOOD AND BIOPRODUCTS PROCESSING; v. 138, p. 12-pg., 2023-02-09.

Abstract

Food supplements from plants are source of many antioxidant substances. Tribulus ter-restris food supplement (TtFS) is highlighted as one of these supplements that has pre-sented potential to prevent damages and diseases caused by oxidative stress. In addition, new formulations that enhance the benefits of food supplement have been developed and metallic nanoparticles, the so-called green synthesis, has been of great interest. UV-visible spectroscopy, energy dispersive spectroscopy (EDS), Zeta potential (ZP), scan-ning transmission electron microscopy (STEM), scanning electron microscope (SEM) were used to characterize the synthesized silver nanoparticles (AgNPs). Antioxidant activity was determined by DPPH radical scavenging, ferric ion reducing power (FRAP); ABTS ra-dical scavenging, and oxygen radical absorption capacity (ORAC-FL). Molecular docking analyses were performed to evaluate molecular interactions. Antioxidant evaluation of TtFS demonstrated 83.6% antioxidant activity (DPPH), 714.82 mu M Trolox Equivalent (TE)/g sample (FRAP), 91.24 mu M TE/g sample (ABTS), and antioxidant activity in ORAC assay. It presented inhibition activity on collagenase (75.33%), elastase (54.04%) and tyrosinase (59.07%). In addition, in silico assays showed an interaction between T. terrestris steroidal saponins and DNA, and the highest DNA binding affinity was observed with protodioscin. Steroidal saponins also presented interaction with all enzymes evaluated. The characterization of AgNPs demonstrated that they were formed as a spherical shape. These AgNPs showed greater protective properties against oxidative stress (85.41% anti-oxidant activity (DPPH) and 957.55 mu M TE/g sample (FRAP)). The results represent an im-portant indicator for the development and discovery of new nanoestructured pharmaceutical and cosmetic formulation using plants and natural products. (c) 2023 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 15/17893-3 - Antioxidant and antiglicante activity of microencapsulation of the extract and saponins obtained from Tribulus terrestris
• Grantee: Célia Cristina Malaguti Figueiredo
• Support Opportunities: Scholarships in Brazil - Master