Comparison of the analytical performance of electrophoresis microchannels fabricated in PDMS, glass, and polyester-toner

Author(s)

Tomazelli Coltro, Wendell Karlos ; Lunte, Susan M. ; Carrilho, Emanuel

• Document type: Journal article
• Source: ELECTROPHORESIS; v. 29, n. 24, p. 10-pg., 2008-12-01.

Abstract

This paper compares the analytical performance of microchannels fabricated in PDMS, glass, and polyester-toner for electrophoretic separations. Glass and PDMS chips were fabricated using well-established photolithographic and replica-molding procedures, respectively. PDMS channels were sealed against three different types of materials: native PDMS, plasma-oxidized PDMS, and glass. Polyester-toner chips were micromachined by a direct-printing process using an office laser printer. All microchannels were fabricated with similar dimensions according to the limitations of the direct-printing process (width/depth 150 mu m/12 mu m). LIF was employed for detection to rule out any losses in separation efficiency due to the detector configuration. Two fluorescent dyes, coumarin and fluorescein, were used as model analytes. Devices were evaluated for the following parameters related to electrophoretic separations: EOF, heat dissipation, injection reproducibility, separation efficiency, and adsorption to channel wall. (AU)

• FAPESP's process: 04/01525-0 - Development of micro devices with manufactured micro electrodes for monitoring biomolecular interactions with oscillometric detection
• Grantee: Wendell Karlos Tomazelli Coltro
• Support Opportunities: Scholarships in Brazil - Doctorate