Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

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Author(s):	Maia Faria, Joice Lara ; Dagnone, Ana Silvia ; Munhoz, Thiago Demarchi ; Joao, Carolina Franchi ; Biscola Pereira, Wanderson Adriano ; Machado, Rosangela Zacarias ; Tinucci-Costa, Mirela Total Authors: 7
Document type:	Journal article
Source:	REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 19, n. 2, p. 5-pg., 2010-04-01.
Abstract
The aim of this study was to compare the detection of Ehrlichta cams morulae and DNA by nPCR in whole blood and spleen aspiration The sample Included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears E cants DNA was detected in 29/40 blood samples We verified that morulae detection is more efficient in cytological preparations from spleen aspiration On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis (AU)