Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum

Author(s)

Colussi, Francieli ; Textor, Larissa C. ; Serpa, Viviane ; Maeda, Roberto Nobuyuki ; Pereira, Nei, Jr. ; Polikarpov, Igor

• Document type: Journal article
• Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 66, p. 4-pg., 2010-09-01.

Abstract

The filamentous fungus Trichoderma harzianum has a considerable cellulolytic activity that is mediated by a complex of enzymes which are essential for the hydrolysis of microcrystalline cellulose. These enzymes were produced by the induction of T. harzianum with microcrystalline cellulose (Avicel) under submerged fermentation in a bioreactor. The catalytic core domain (CCD) of cellobiohydrolase I (CBHI) was purified from the extracellular extracts and submitted to robotic crystallization. Diffraction-quality CBHI CCD crystals were grown and an X-ray diffraction data set was collected under cryogenic conditions using a synchrotron-radiation source. (AU)

• FAPESP's process: 09/05349-6 - Expression and purification of cellobiohydrolase I from the filamentous fungus Trichoderma harzianum
• Grantee: Bruno Luan Soares Paula de Mello
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
• Grantee: Igor Polikarpov
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 07/08706-9 - Molecular and Structural Studies of Cellulitic Complex Enzymes of Fungi Trichoderma harzianum with Potencial of Enzymatic Transformation of Biomass
• Grantee: Viviane Isabel Serpa
• Support Opportunities: Scholarships in Brazil - Doctorate