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Influence of an accessory domain in biochemical and structural characteristics of the cellulase CelE2

Grant number: 16/01926-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2016
Effective date (End): February 28, 2018
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Fábio Márcio Squina
Grantee:Agnes Cristina Pimentel
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
Associated research grant:14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models, AP.BIOEN.TEM


Different physicochemical and enzymatic treatments are applied in order to overcome the recalcitrance of plant cell wall to degradation. Glycosyl hydrolases (GHs) integrate a class of enzymes widely used in enzymatic process since they perform the hydrolysis of glycosidic bonds in a wide variety of polysaccharides. Apart from the catalytic domains, several glycosyl hydrolases contain accessories domains that can impact on biochemical and structural features. A previous study demonstrated that the cellulase CelE2, from metagenomic origin, contains a N-terminal domain characteristic of GH5 family and a second domain in the C-terminal region, displaying identity to Calx-beta domain, which is described as a cytoplasmic domain involved in exchange of ions sodium and calcium. Preliminary studies suggested the influence of C-terminal domain in CelE2 enzymatic activity. This domain had already been identified in other glycosyl hydrolases, however no clear function for this domain have been reported so far. In this context, the main objective of this project is to elucidate the influence of the C-terminal domain on CelE2 enzymatic activity. For this, biochemical and structural features will be evaluated through circular dichroism, small angle X-ray scattering experiments, as well as crystallographic and X-ray diffraction analysis. Furthermore, studies will be conduct to evaluate the ability of the C-terminal domain to bind to carbohydrates since the comparison with the dbCan database showed identity, although with low significance, with carbohydrate binding domains of family 35. Finally, biochemical and structural information will be used as input data for molecular dynamics studies aiming to access in details the probable molecular interactions occurring between the two domains of cellulase CelE2. Currently, there is no detailed information about the modular architecture of CelE2 and the elucidation of the hole of its C-terminal domain can provide support for the rational design of glycosyl hydrolases. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIMENTEL, AGNES C.; EMATSU, GABRIELA C. G.; LIBERATO, MARCELO V.; PAIXAO, DOUGLAS A. A.; FRANCO CAIRO, JOAO PAULO L.; MANDELLI, FERNANDA; TRAMONTINA, ROBSON; GANDIN, CESAR A.; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO M.; et al. Biochemical and biophysical properties of a metagenome-derived GH5 endoglucanase displaying an unconventional domain architecture. International Journal of Biological Macromolecules, v. 99, p. 384-393, . (14/06923-6, 14/04105-4, 16/01926-2, 10/11469-1, 14/12861-3, 08/58037-9)
PIMENTEL, AGNES C.; LIBERATO, MARCELO V.; CAIRO, JOAO PAULO L. FRANCO; TOMAZETTO, GEIZECLER; GANDIN, CESAR A.; NETO, MARIO DE OLIVEIRA; ALVAREZ, THABATA M.; SQUINA, FABIO M.. Structural and functional insights of the catalytic GH5 and Calx-beta domains from the metagenome-derived endoglucanase CelE2. Enzyme and Microbial Technology, v. 165, p. 10-pg., . (16/01926-2, 15/50590-4, 20/05784-3, 14/04105-4, 10/11469-1, 16/09950-0, 15/23279-6)
TOMAZETTO, GEIZECLER; PIMENTEL, AGNES C.; WIBBERG, DANIEL; DIXON, NEIL; SQUINA, FABIO M.. Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium. Applied and Environmental Microbiology, v. 86, n. 18, . (16/01926-2, 18/23826-5, 15/50590-4, 15/23279-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PIMENTEL, Agnes Cristina. Biochemical and structural features of the catalytic domain of cellulase CelE2 and its relation with the Calx-beta accessory domain. 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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