Isolation and antimicrobial activity of Streptomyces tubercidicus against pathogenic bacteria and fungi

Author(s)

Priscila Ratti, R. ; Toledo Piza, A. C. M. ; Granato, A. C. ; Hokka, C. O. ; Paiva de Sousa, C. ; MendezVilas, A

• Document type: Journal article
• Source: MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS; v. N/A, p. 3-pg., 2011-01-01.

Abstract

Streptomyces spp. produces many secondary metabolites, including antibiotics. The substances formation is coupled with the onset of development of the microorganism and the search of new substances is important. The aim of this work was to evaluate the bioactivity of endophytic Streptomyces tubercidicus isolated from Solanum lycocarpum St. Hill, a typical Brazilian tropical savannah tree to test its inhibitory capability against pathogenic bacteria and fungi. S. tubercidicus was cultivated in ISP2A and submitted to antibiosis test against S. aureus (ATCC 29213), P. aeruginosa (ATCC 27853), E. coli (ATCC 25922) and C. albicans (ATCC 10231). S. tubercidicus presented antibiosis against S. aureus (35 mm), E. coli (40 mm) and C. albicans (19 mm), but failed to inhibit P. aeruginosa. Purification of crude extract of S. tubercidicus with permeation in Sephadex L20 gel methanol revealed two compounds with bioactivity against S. aureus and E. coli. (AU)

• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants