| Full text | |
| Author(s): |
Alves, Raquel Melo
;
Feliciano, Patricia Rosa
;
Sampaio, Suely Vilela
;
Nonato, Maria Cristina
Total Authors: 4
|
| Document type: | Journal article |
| Source: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 67, p. 4-pg., 2011-04-01. |
| Abstract | |
Despite the valuable contributions of robotics and high-throughput approaches to protein crystallization, the role of an experienced crystallographer in the evaluation and rationalization of a crystallization process is still crucial to obtaining crystals suitable for X-ray diffraction measurements. In this work, the difficult task of crystallizing the flavoenzyme l-amino-acid oxidase purified from Bothrops atrox snake venom was overcome by the development of a protocol that first required the identification of a non-amorphous precipitate as a promising crystallization condition followed by the implementation of a methodology that combined crystallization in the presence of oil and seeding techniques. Crystals were obtained and a complete data set was collected to 2.3 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 73.64, b = 123.92, c = 105.08 A, beta = 96.03 degrees. There were four protein subunits in the asymmetric unit, which gave a Matthews coefficient V (M) of 2.12 A3 Da-1, corresponding to 42% solvent content. The structure has been solved by molecular-replacement techniques. (AU) | |
| FAPESP's process: | 05/54855-0 - Animal toxins: structure, function and biotechnological applications |
| Grantee: | Suely Vilela |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/10454-3 - Studies of the correlation between structure and function of the enzyme fumarate hydratase in Leishmania major |
| Grantee: | Patrícia Rosa Feliciano |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 07/51700-1 - Análise da estrutura tridimensional da L-aminoácido oxidase de Bothops atrox e suas interações in vitro e in vivo |
| Grantee: | Raquel de Melo Alves Paiva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |