Determination of a method for extraction of coenzyme Q(10) in human plasma: optimization of the use of surfactants and other variables

Author(s)

Ferreiro-Barros, Claudia Cristina ; Sugawara, Eduardo Kinio ; Sanches, Livia Rentas

• Document type: Journal article
• Source: Einstein (São Paulo); v. 10, n. 2, p. 6-pg., 2012-04-01.

Abstract

Objective: To establish a routine for the extraction of the total levels of CoQ 10 in human plasma through the Ultra High Performance Liquid Chromatography (UHPLC). Methods: Two extraction protocols were tested: a) methanol: hexane and b) 1-propanol. The following parameters were analyzed: extraction temperature (19 degrees C and 4 degrees C), extraction tubes (glass and polypropylene), and surfactants (SDS, Triton X-100, Tween-20) at different concentrations, i.e., 1%, 3%, 5% and 10%. Results: The results showed that the method of extraction of CoQ(10) in a sample of human plasma at 4 degrees C, using solvents methanol: hexane (85: 15, v/v) in the presence of surfactant Tween-20 at 3% and polypropylene tubes showed better efficiency and reproducibility when compared to the method with 1-propanol. Conclusion: By the analyses performed, it was possible to observe that the addition of the surfactant Tween-20 promoted an increase in the recovery of CoQ(10) by the methanol: hexane extraction method. This method showed good reproducibility, with a low coefficient of variation and high sensitivity, since CoQ(10) was detected in samples of plasma of a control individual using a UV-type detector. The use of UHPLC equipment allowed a total analysis with total run time of 3.5 minutes, enabling the rapid achievement of results, considered mandatory for laboratory routines. (AU)

• FAPESP's process: 10/51924-0 - Identification of patients with metabolic defect in coenzyme Q10
• Grantee: Claudia Cristina Ferreiro de Barros
• Support Opportunities: Regular Research Grants